Linked Life Data

19941388

Source:http://linkedlifedata.com/resource/pubmed/id/19941388

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-11-27
pubmed:abstractText
It has been shown that Ha127 in the genome of Helicoverpa armigera nucleopolyhedrovirus (HaNPV) has homologs in some other baculoviruses and encodes a putative protein of 192 aa. In this study, a sequence analysis showed the transcription initiation site in Ha127 gene at nts 188 upstream of the translation initiation codon ATG and a potential leucine zipper motif at aa 34-55 in the corresponding protein. Ha127 transcripts were detected in HaNPV-infected HzAM1 cells at 18-72 hrs post infection ( p.i.) by RT-PCR, while the corresponding protein was found at 24-72 hrs p.i. by Western blot analysis suggesting that Ha127 is a late gene product. The size of detected Ha127 protein was about 28 K, a larger value than the predicted 22.6 K indicating a post-translational modification. Immunofluorescence assay of HzAM1 cells infected with HaNPV and Ha127-EGFP expression showed that Ha127 protein was localized in the nucleus. In summary, these data suggested that Ha127 was a functional ORF that might play a role in the nucleus during the late or very late gene expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0001-723X
pubmed:author
pubmed:issnType
Print
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
247-53
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Characterization of ORF127 of Helicoverpa armigera nucleopolyhedrovirus.
pubmed:affiliation
Institute of Life Science, Jiangsu University, PR China. gzh762677@ujs.edu.cn
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't