Linked Life Data

19940244

Source:http://linkedlifedata.com/resource/pubmed/id/19940244

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
50
pubmed:dateCreated
2009-12-18
pubmed:abstractText
Starch defines an insoluble semicrystalline form of storage polysaccharides restricted to Archaeplastida (red and green algae, land plants, and glaucophytes) and some secondary endosymbiosis derivatives of the latter. While green algae and land-plants store starch in plastids by using an ADP-glucose-based pathway related to that of cyanobacteria, red algae, glaucophytes, cryptophytes, dinoflagellates, and apicomplexa parasites store a similar type of polysaccharide named floridean starch in their cytosol or periplast. These organisms are suspected to store their floridean starch from UDP-glucose in a fashion similar to heterotrophic eukaryotes. However, experimental proof of this suspicion has never been produced. Dinoflagellates define an important group of both photoautotrophic and heterotrophic protists. We now report the selection and characterization of a low starch mutant of the heterotrophic dinoflagellate Crypthecodinium cohnii. We show that the sta1-1 mutation of C. cohnii leads to a modification of the UDP-glucose-specific soluble starch synthase activity that correlates with a decrease in starch content and an alteration of amylopectin structure. These experimental results validate the UDP-glucose-based pathway proposed for floridean starch synthesis.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19940244-10467041, http://linkedlifedata.com/resource/pubmed/commentcorrection/19940244-15785854, http://linkedlifedata.com/resource/pubmed/commentcorrection/19940244-16757743, http://linkedlifedata.com/resource/pubmed/commentcorrection/19940244-17248757, http://linkedlifedata.com/resource/pubmed/commentcorrection/19940244-18055913, http://linkedlifedata.com/resource/pubmed/commentcorrection/19940244-18093994, http://linkedlifedata.com/resource/pubmed/commentcorrection/19940244-18245855, http://linkedlifedata.com/resource/pubmed/commentcorrection/19940244-18838391, http://linkedlifedata.com/resource/pubmed/commentcorrection/19940244-4851938, http://linkedlifedata.com/resource/pubmed/commentcorrection/19940244-4852153, http://linkedlifedata.com/resource/pubmed/commentcorrection/19940244-560475, http://linkedlifedata.com/resource/pubmed/commentcorrection/19940244-8150278, http://linkedlifedata.com/resource/pubmed/commentcorrection/19940244-9390431, http://linkedlifedata.com/resource/pubmed/commentcorrection/19940244-9730163, http://linkedlifedata.com/resource/pubmed/commentcorrection/19940244-9848667
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1091-6490
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
106
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
21126-30
pubmed:dateRevised
2010-9-27
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Genetic dissection of floridean starch synthesis in the cytosol of the model dinoflagellate Crypthecodinium cohnii.
pubmed:affiliation
Unité de Glycobiologie Structurale et Fonctionnelle, Unité Mixte de Recherche 8576, Centre National de la Recherche Scientifique and Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq Cedex, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't