Linked Life Data

19938181

Source:http://linkedlifedata.com/resource/pubmed/id/19938181

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2009-11-26
pubmed:abstractText
An ultrafast electrophoresis method was developed to have an insight into the interaction of E. coli, DH5alpha, with an antibiotic gentamycin. The method used micro-video to visually measure the migration of bacteria, rather than molecules, within several micrometers, taking a time down to 0.04 s. Precise bacterial mobility was measured at the electric field strength of 42.5-62.5 V/cm, with a mobility deviation of less than 1.7% compared with a common CE method. The bacterial mobility measured this way was found to have a distribution increasing with the electric field strength applied, but the averaged mobility kept constant or independent of the electric field strength. As indicated by the bacterial mobility measured, DH5alpha experienced four stages during interaction with the antibiotic gentamycin, namely, the outer surface adsorption equilibration, uptake or transferring of adsorbed gentamycin through cellular membrane, destruction of the membrane, and death of the bacteria. The bacterial outer surface adsorption of gentamycin was found to have duration up to about 5 min that decreased reversely with the concentration of gentamycin added. This proves that the activity of antibiotic gentamycin is dose-dependent.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1522-2683
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3884-90
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Insight into the interaction of E. coli with gentamycin by ultrafast electrophoresis achieved within the microscopic visual field.
pubmed:affiliation
Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, P. R. China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't