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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1991-3-15
|
| pubmed:abstractText |
A biologically active preparation of murine recombinant interleukin-1 beta (mIL-1 beta) from Escherichia coli cell lysates contained tow forms of mIL-1 beta with pI 8.7 and pI 8.1, respectively. Treatment with 0.1 M Tris, pH 8.5, at 37 degrees C for 35 h converted the pI 8.7 form to the pI 8.1 form by the selective deamidation of an asparagine residue (Asn149) in the mIL-1 beta molecule. Deamidated mIL-1 beta had 3- to 5-fold lower co-mitogenic activity and receptor affinity than the unmodified form.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0014-5793
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
14
|
| pubmed:volume |
278
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
98-102
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1993481-Amides,
pubmed-meshheading:1993481-Amino Acid Sequence,
pubmed-meshheading:1993481-Animals,
pubmed-meshheading:1993481-Asparagine,
pubmed-meshheading:1993481-Chromatography, Ion Exchange,
pubmed-meshheading:1993481-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1993481-Interleukin-1,
pubmed-meshheading:1993481-Mice,
pubmed-meshheading:1993481-Molecular Sequence Data,
pubmed-meshheading:1993481-Recombinant Proteins,
pubmed-meshheading:1993481-Structure-Activity Relationship
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Reduction of biological activity of murine recombinant interleukin-1 beta by selective deamidation at asparagine-149.
|
| pubmed:affiliation |
Central Research Division, Pfizer Inc., Groton, CT 06340.
|
| pubmed:publicationType |
Journal Article
|