Linked Life Data

19933861

Source:http://linkedlifedata.com/resource/pubmed/id/19933861

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2009-12-16
pubmed:abstractText
We have recently reported that the sublingual (s.l.) mucosa is an efficient site for inducing systemic and mucosal immune responses. In this study, the potential of s.l. immunization to induce remote Ab responses and CD8(+) cytotoxic responses in the female genital tract was examined in mice by using a nonreplicating Ag, OVA, and cholera toxin (CT) as an adjuvant. Sublingual administration of OVA and CT induced Ag-specific IgA and IgG Abs in blood and in cervicovaginal secretions. These responses were associated with large numbers of IgA Ab-secreting cells (ASCs) in the genital mucosa. Genital ASC responses were similar in magnitude and isotype distribution after s.l., intranasal, or vaginal immunization and were superior to those seen after intragastric immunization. Genital, but not blood or spleen, IgA ASC responses were inhibited by treatment with anti-CCL28 Abs, suggesting that the chemokine CCL28 plays a major role in the migration of IgA ASC progenitors to the reproductive tract mucosa. Furthermore, s.l. immunization with OVA induced OVA-specific effector CD8(+) cytolytic T cells in the genital mucosa, and these responses required coadministration of the CT adjuvant. Furthermore, s.l. administration of human papillomavirus virus-like particles with or without the CT adjuvant conferred protection against genital challenge with human papillomavirus pseudovirions. Taken together, these findings underscore the potential of s.l. immunization as an efficient vaccination strategy for inducing genital immune responses and should impact on the development of vaccines against sexually transmitted diseases.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1550-6606
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
183
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7851-9
pubmed:meshHeading
pubmed-meshheading:19933861-Adjuvants, Immunologic, pubmed-meshheading:19933861-Administration, Sublingual, pubmed-meshheading:19933861-Animals, pubmed-meshheading:19933861-Antibodies, Bacterial, pubmed-meshheading:19933861-Antibodies, Viral, pubmed-meshheading:19933861-Antibody-Producing Cells, pubmed-meshheading:19933861-Cell Differentiation, pubmed-meshheading:19933861-Cells, Cultured, pubmed-meshheading:19933861-Cholera Toxin, pubmed-meshheading:19933861-Disease Models, Animal, pubmed-meshheading:19933861-Female, pubmed-meshheading:19933861-Human papillomavirus 16, pubmed-meshheading:19933861-Mice, pubmed-meshheading:19933861-Mice, Inbred BALB C, pubmed-meshheading:19933861-Mice, Inbred C57BL, pubmed-meshheading:19933861-Mucous Membrane, pubmed-meshheading:19933861-Ovalbumin, pubmed-meshheading:19933861-Papillomavirus Infections, pubmed-meshheading:19933861-T-Lymphocytes, Cytotoxic, pubmed-meshheading:19933861-Uterine Cervical Neoplasms, pubmed-meshheading:19933861-Virion
pubmed:year
2009
pubmed:articleTitle
Sublingual immunization with nonreplicating antigens induces antibody-forming cells and cytotoxic T cells in the female genital tract mucosa and protects against genital papillomavirus infection.
pubmed:affiliation
Laboratory Sciences Division, International Vaccine Institute, Seoul, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't