Linked Life Data

19933856

Source:http://linkedlifedata.com/resource/pubmed/id/19933856

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2009-12-16
pubmed:abstractText
Pulmonary clearance of the encapsulated yeast Cryptococcus neoformans is associated with the CCR2-mediated accumulation of lung dendritic cells (DC) and the development of a T1 adaptive immune response. The objective of this study was to identify the circulating DC precursor(s) responsible for this large increase in lung DC numbers. An established murine model was used to evaluate putative DC precursors in the blood, bone marrow, and lungs of CCR2(+/+) mice and CCR2(-/-) mice throughout a time course following infection with C. neoformans. Results demonstrate that numbers of Ly-6C(high) monocytes increased in parallel in the peripheral blood and lungs of CCR(+/+) mice, whereas CD11c(+) MHC class II(+) pre-DC were 10-fold less prevalent in the peripheral blood and did not differ between the two strains. Accumulation of Ly-6C(high) monocytes correlated with a substantial increase in the numbers of CD11b(+) DC in the lungs of infected CCR2(+/+) mice. Comparative phenotypic analysis of lung cells recovered in vivo suggests that Ly-6C(high) monocytes differentiate into CD11b(+) DC in the lung; differentiation is associated with up-regulation of costimulatory molecules and decreased Ly-6C expression. Furthermore, in vitro experiments confirmed that Ly-6C(high) monocytes differentiate into CD11b(+) DC. Accumulation of Ly-6C(high) monocytes and CD11b(+) DC was not attributable to their proliferation in situ. We conclude that the CCR2-mediated accumulation of CD11b(+) DC in the lungs of Cryptococcus-infected mice is primarily attributable to the continuous recruitment and differentiation of Ly-6C(high) monocytes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1550-6606
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
183
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8044-53
pubmed:meshHeading
pubmed-meshheading:19933856-Animals, pubmed-meshheading:19933856-Antigens, CD11b, pubmed-meshheading:19933856-Antigens, Ly, pubmed-meshheading:19933856-Cell Count, pubmed-meshheading:19933856-Cell Differentiation, pubmed-meshheading:19933856-Cell Movement, pubmed-meshheading:19933856-Cell Proliferation, pubmed-meshheading:19933856-Cryptococcosis, pubmed-meshheading:19933856-Cryptococcus neoformans, pubmed-meshheading:19933856-Cytokinesis, pubmed-meshheading:19933856-Dendritic Cells, pubmed-meshheading:19933856-Female, pubmed-meshheading:19933856-Lung, pubmed-meshheading:19933856-Lung Diseases, Fungal, pubmed-meshheading:19933856-Mice, pubmed-meshheading:19933856-Mice, Inbred BALB C, pubmed-meshheading:19933856-Mice, Knockout, pubmed-meshheading:19933856-Monocytes, pubmed-meshheading:19933856-Receptors, CCR2, pubmed-meshheading:19933856-Stem Cells
pubmed:year
2009
pubmed:articleTitle
Accumulation of CD11b+ lung dendritic cells in response to fungal infection results from the CCR2-mediated recruitment and differentiation of Ly-6Chigh monocytes.
pubmed:affiliation
Pulmonary Section, Medical Service, Ann Arbor Veterans Affairs Health System, Department of Veterans Affairs Health System, Ann Arbor, MI 48105, USA. oster@umich.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural