Linked Life Data

19933265

Source:http://linkedlifedata.com/resource/pubmed/id/19933265

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-1-18
pubmed:databankReference
pubmed:abstractText
To identify the function of genes that regulate the processing of proglutelin, we performed an analysis of glup3 mutants, which accumulates excess amounts of proglutelin and lack the vacuolar processing enzyme (VPE). VPE activity in developing seeds from glup3 lines was reduced remarkably compared with the wild type. DNA sequencing of the VPE gene in glup3 mutants revealed either amino acid substitutions or the appearance of a stop codon within the coding region. Microscopic observations showed that alpha-globulin and proglutelin were distributed homogeneously within glup3 protein storage vacuoles (PSVs), and that glup3 PSVs lacked the crystalline lattice structure typical of wild-type PSVs. This suggests that the processing of proglutelin by VPE in rice is essential for proper PSV structure and compartmentalization of storage proteins. Growth retardation in glup3 seedlings was also observed, indicating that the processing of proglutelin influences early seedling development. These findings indicate that storage of glutelin in its mature form as a crystalline structure in PSVs is required for the rapid use of glutelin as a source of amino acids during early seedling development. In conclusion, VPE plays an important role in the formation of protein crystalline structures in PSVs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1471-9053
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
38-46
pubmed:meshHeading
pubmed-meshheading:19933265-Amino Acids, pubmed-meshheading:19933265-Base Sequence, pubmed-meshheading:19933265-Codon, Terminator, pubmed-meshheading:19933265-Crystallization, pubmed-meshheading:19933265-Crystallography, X-Ray, pubmed-meshheading:19933265-Cysteine Endopeptidases, pubmed-meshheading:19933265-Fluorescent Antibody Technique, pubmed-meshheading:19933265-Gene Expression Regulation, Plant, pubmed-meshheading:19933265-Genes, Plant, pubmed-meshheading:19933265-Glutens, pubmed-meshheading:19933265-Microscopy, Electron, Transmission, pubmed-meshheading:19933265-Molecular Sequence Data, pubmed-meshheading:19933265-Mutation, pubmed-meshheading:19933265-Oryza sativa, pubmed-meshheading:19933265-Protein Structure, Secondary, pubmed-meshheading:19933265-Seeds, pubmed-meshheading:19933265-Vacuoles
pubmed:year
2010
pubmed:articleTitle
Vacuolar processing enzyme plays an essential role in the crystalline structure of glutelin in rice seed.
pubmed:affiliation
Faculty of Agriculture, Kyushu University, Fukuoka, Japan. kumamaru@agr.kyushu-u.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't