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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0019704,
umls-concept:C0033684,
umls-concept:C0042776,
umls-concept:C0185117,
umls-concept:C0205374,
umls-concept:C0302133,
umls-concept:C0453108,
umls-concept:C0525037,
umls-concept:C0598496,
umls-concept:C0996935,
umls-concept:C1413292,
umls-concept:C1423526,
umls-concept:C2003905,
umls-concept:C2753500,
umls-concept:C2911684
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pubmed:dateCreated |
2009-12-1
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pubmed:abstractText |
In recent years, different HIV antigens have been successfully expressed in plants by either stable transformation or transient expression systems. Among HIV proteins, Nef is considered a promising target for the formulation of a multi-component vaccine due to its implication in the first steps of viral infection. Attempts to express Nef as a single protein product (not fused to a stabilizing protein) in transgenic plants resulted in disappointingly low yields (about 0.5% of total soluble protein). In this work we describe a transient expression system based on co-agroinfiltration of plant virus gene silencing suppressor proteins in Nicotiana benthamiana, followed by a two-step affinity purification protocol of plant-derived Nef.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-10892807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-11335175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-11457942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-11876469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-12021328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-12151436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-12502820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-12609035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-17049679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-17086054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-17177796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-17206371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-17669207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-18346095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-18444969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-18573204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-18676047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-18775971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-18793269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-19135558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-19140023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-19169897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-19548344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-19548345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-19588264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19930574-8021582
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1472-6750
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
96
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pubmed:dateRevised |
2010-9-28
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pubmed:meshHeading |
pubmed-meshheading:19930574-Chromatography, Affinity,
pubmed-meshheading:19930574-Gene Expression Regulation, Plant,
pubmed-meshheading:19930574-Genetic Engineering,
pubmed-meshheading:19930574-Mass Spectrometry,
pubmed-meshheading:19930574-RNA, Small Interfering,
pubmed-meshheading:19930574-RNA Interference,
pubmed-meshheading:19930574-Tobacco,
pubmed-meshheading:19930574-Tombusvirus,
pubmed-meshheading:19930574-Viral Core Proteins,
pubmed-meshheading:19930574-nef Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2009
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pubmed:articleTitle |
High-level HIV-1 Nef transient expression in Nicotiana benthamiana using the P19 gene silencing suppressor protein of Artichoke Mottled Crinckle Virus.
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pubmed:affiliation |
Centro Ricerche Casaccia, Via Anguillarese 301, I-00123, Rome, Italy. raffaele.lombardi@enea.it
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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