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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2010-2-9
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pubmed:abstractText |
Francisella tularensis induces apoptosis within macrophages but the temporal and spatial modulation through activation of caspase-1, caspase-3, and the anti-apoptosis nuclear transcription factor B (NF-kappaB) is not known. Whether escape of the bacteria into the cytosol is sufficient and/or essential for activation of NF-kappaB is not known. Our results show that F. tularensis subsp. novicida induces sustained nuclear translocation of NF-kappaB at early time points after infection of human monocytes derived macrophages (hMDMs). The sustained nuclear translocation of NF-kappaB is defective in the iglC mutant that fails to escape into the cytosol of macrophages. Nuclear translocation of NF-kappaB by the wild type strain is abolished upon treatment with the NF-kappaB inhibitor caffein acid phenyl ester. While the wild type strain triggers caspase-3 and caspase-1 activation by 6 h post-infection the iglC mutant is defective in triggering both caspases. In hMDMs treated with the apoptosis-inducing agent, staurosporin, there is an induction of cell death in the iglC mutant-infected macrophages despite reduced frequency of caspase-1 and caspase-3 activity. The wt-infected macrophages are resistant to cell death-induced agent. We conclude that although caspase-1 and capsase-3 are triggered within F. tularensis-infected hMDMs during early stages of infection, cell death is delayed, which is correlated with simultaneous activation of NF-kappaB.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-10200555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-10837071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-11207583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-11402018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-11931755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-12542469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-12966164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-14500514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-14644198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-14680699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-15010524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-15519043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-16230474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-16356719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-16373510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-16622218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-16911566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-1692676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-17056562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-17186029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-17372002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-17438033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-18390995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-18474647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-8799159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-9539792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19925880-9733516
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1769-714X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2009 Elsevier Masson SAS. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
126-34
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pubmed:dateRevised |
2011-7-22
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pubmed:meshHeading |
pubmed-meshheading:19925880-Apoptosis,
pubmed-meshheading:19925880-Apoptosis Regulatory Proteins,
pubmed-meshheading:19925880-Caspase 1,
pubmed-meshheading:19925880-Caspase 3,
pubmed-meshheading:19925880-Enzyme Activation,
pubmed-meshheading:19925880-Francisella tularensis,
pubmed-meshheading:19925880-Gene Expression Regulation, Bacterial,
pubmed-meshheading:19925880-Humans,
pubmed-meshheading:19925880-Macrophages,
pubmed-meshheading:19925880-NF-kappa B,
pubmed-meshheading:19925880-Signal Transduction
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pubmed:year |
2010
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pubmed:articleTitle |
Regulation of apoptosis and anti-apoptosis signalling by Francisella tularensis.
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pubmed:affiliation |
Department of Microbiology and Parasitology, University of Rijeka, Medical Faculty, Brace Branchetta 20, 51000 Rijeka, Croatia. marina@medri.hr
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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