Source:http://linkedlifedata.com/resource/pubmed/id/19913604
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2010-2-15
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pubmed:abstractText |
Cisplatin (cis-diamminedichloroplatinum II, CDDP) is a chemotherapeutic agent that induces nephrotoxicity associated with oxidative/nitrosative stress. Sulforaphane (SFN) is an isothiocyanate produced by the enzymatic action of myrosinase on glucorophanin, a glucosinolate contained in cruciferous vegetables. SFN is able to induce cytoprotective enzymes through the transcription factor Nrf2. The purpose of this study was to evaluate whether SFN induces a cytoprotective effect on the CDDP-induced nephrotoxicity. Preincubation of LLC-PK1 cells with 0.5-5 microM SFN by 24 h was able to prevent, in a concentration-dependent way, CDDP-induced cell death. Immunofluorescent staining confirmed the nuclear translocation of Nrf2 after treatment with SFN. In the in vivo studies, CDDP was given to Wistar rats as a sole i.p. injection at a dose of 7.5 mg/kg. SFN (500 microg/kg i.v.) was given two times (24 h before and 24 after CDDP-injection). Animals were killed three days after CDDP-injection. SFN attenuated CDDP-induced renal dysfunction, structural damage, oxidative/nitrosative stress, glutathione depletion, enhanced urinary hydrogen peroxide excretion and the decrease in antioxidant enzymes (catalase, glutathione peroxidase and glutathione-S-transferase). The renoprotective effect of SFN on CDDP-induced nephrotoxicity was associated with the attenuation in oxidative/nitrosative stress and the preservation of antioxidant enzymes.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anticarcinogenic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Cisplatin,
http://linkedlifedata.com/resource/pubmed/chemical/NF-E2-Related Factor 2,
http://linkedlifedata.com/resource/pubmed/chemical/Thiocyanates,
http://linkedlifedata.com/resource/pubmed/chemical/sulforafan
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1879-3169
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pubmed:author |
pubmed-author:Calderón-OliverMarielM,
pubmed-author:FrancoMarthaM,
pubmed-author:Guerrero-BeltránCarlos EnriqueCE,
pubmed-author:Martínez-MartínezClaudia MaríaCM,
pubmed-author:Medina-CamposOmar NON,
pubmed-author:Ortiz-VegaKarla MarianaKM,
pubmed-author:Pedraza-ChaverriJoséJ,
pubmed-author:Sánchez-GonzálezDolores JavierDJ,
pubmed-author:TapiaEdiliaE
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pubmed:copyrightInfo |
Copyright 2009 Elsevier Ireland Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
192
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
278-85
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pubmed:meshHeading |
pubmed-meshheading:19913604-Active Transport, Cell Nucleus,
pubmed-meshheading:19913604-Animals,
pubmed-meshheading:19913604-Anticarcinogenic Agents,
pubmed-meshheading:19913604-Antineoplastic Agents,
pubmed-meshheading:19913604-Cell Death,
pubmed-meshheading:19913604-Cell Nucleus,
pubmed-meshheading:19913604-Cell Survival,
pubmed-meshheading:19913604-Cisplatin,
pubmed-meshheading:19913604-Dose-Response Relationship, Drug,
pubmed-meshheading:19913604-Kidney,
pubmed-meshheading:19913604-LLC-PK1 Cells,
pubmed-meshheading:19913604-Male,
pubmed-meshheading:19913604-NF-E2-Related Factor 2,
pubmed-meshheading:19913604-Oxidative Stress,
pubmed-meshheading:19913604-Rats,
pubmed-meshheading:19913604-Rats, Wistar,
pubmed-meshheading:19913604-Swine,
pubmed-meshheading:19913604-Thiocyanates
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pubmed:year |
2010
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pubmed:articleTitle |
Sulforaphane protects against cisplatin-induced nephrotoxicity.
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pubmed:affiliation |
Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, Edificio F, Mexico City, Mexico.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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