Linked Life Data

19910678

Source:http://linkedlifedata.com/resource/pubmed/id/19910678

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5-6
pubmed:dateCreated
2009-11-13
pubmed:abstractText
Uncoupling protein-2 (UCP2) and uncoupling protein-5 (UCP5) are ion carriers located in the inner mitochondrial membrane that mediate a regulated discharge of the proton gradient generated by the respiratory chain and are possibly involved in the protection against free radical production. Mitochondrial dysfunction is involved in the pathogenesis of colon cancer for which UCP2 has been proposed as a potential therapeutic target. The object of this study was to investigate the possible effects of mitochondrial dysfunction and oxidative stress on the mitochondrial content of UCP2 and UCP5 in colonic cells. Proliferation rate/index, antioxidant systems, reactive oxygen species, oxidative damage, mitochondrial markers and protein expression of UCP2 and UCP5 were analysed in non-metastatic HT-29 cells, metastatic SW-620 cells, and in a HT-29 differentiated derivative (Glu-R). In this study, we found a clear relationship between mitochondrial dysfunction in colonic epithelial cells and the levels of UCP2 and UCP5 proteins. Furthermore, hydrogen peroxide was responsible for the coordinated activation of the antioxidant response and the increase of UCP5 protein levels. Our findings suggest that uncoupling activity could act as a local feedback mechanism to limit the enhanced steady-state H(2)O(2) production in dysfunctional mitochondria of colonic cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1421-9778
pubmed:author
pubmed:copyrightInfo
2009 S. Karger AG, Basel.
pubmed:issnType
Electronic
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
379-90
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Hydrogen peroxide regulates the mitochondrial content of uncoupling protein 5 in colon cancer cells.
pubmed:affiliation
Departament de Biologia Fonamental i Ciències de la Salut, Institut Universitari d'Investigació en Ciències de la Salut, Universitat de les Illes Balears, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't