Source:http://linkedlifedata.com/resource/pubmed/id/19909474
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9-10
|
| pubmed:dateCreated |
2009-11-13
|
| pubmed:abstractText |
An EDTA-blood sample from a cow without clinical signs, which gave early birth to a newborn calf that died soon after delivery, was shown to be positive for bluetongue virus (BTV)-RNA using a group-specific real-time RT-PCR (RT-qPCR). In-house serotype-specific RT-qPCR assays for bluetongue virus serotype 1 (BTV-1), -6 and -8 all gave negative results. Subsequent assays were carried out using conventional (gel-based) RT-PCR primers for all 25 BTV serotypes and only two primer sets, both specific for BTV-11, gave bands of the expected size. The cDNAs generated were sequenced and comparisons of the genome segment 2 sequence with that of the modified 'live' vaccine strain of BTV-11 from South Africa showed 100% identity. A survey of all ruminants in a 1-km area around the first positive farm using a BTV-11 serotype-specific RT-qPCR revealed five other holdings with in total nine BTV-11 positive animals. A cross-sectional monitoring of dairy cattle in Belgium showed an overall prevalence of 3.8% on herd level and 0.2% on animal level. A BTV-11 has been introduced into the Belgian cattle herd during the 2008 vector season. The source of the infection and the way by which the virus was introduced are unknown.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
1865-1682
|
| pubmed:author |
pubmed-author:BattenCC,
pubmed-author:Bin-TarifAA,
pubmed-author:BréardEE,
pubmed-author:De ClercqKK,
pubmed-author:De LeeuwII,
pubmed-author:DuboisEE,
pubmed-author:Gastaldi-ThiéryCC,
pubmed-author:HenstockMM,
pubmed-author:HooyberghsJJ,
pubmed-author:HoudartPP,
pubmed-author:MaasTT,
pubmed-author:MaazBB,
pubmed-author:MertensPP,
pubmed-author:NomikouKK,
pubmed-author:OuraCC,
pubmed-author:PotgieterA CAC,
pubmed-author:RaemaekersMM,
pubmed-author:RiocreuxFF,
pubmed-author:SandersAA,
pubmed-author:ShawAA,
pubmed-author:VandemeulebrouckeEE,
pubmed-author:VandenbusscheFF,
pubmed-author:VanderstedeYY,
pubmed-author:VerheydenBB,
pubmed-author:WrightI MIM,
pubmed-author:ZientaraSS
|
| pubmed:issnType |
Electronic
|
| pubmed:volume |
56
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
355-61
|
| pubmed:meshHeading |
pubmed-meshheading:19909474-Animals,
pubmed-meshheading:19909474-Antibodies, Viral,
pubmed-meshheading:19909474-Belgium,
pubmed-meshheading:19909474-Bluetongue,
pubmed-meshheading:19909474-Bluetongue virus,
pubmed-meshheading:19909474-Cattle,
pubmed-meshheading:19909474-Cattle Diseases,
pubmed-meshheading:19909474-Cross-Sectional Studies,
pubmed-meshheading:19909474-Dairying,
pubmed-meshheading:19909474-Female,
pubmed-meshheading:19909474-Population Surveillance,
pubmed-meshheading:19909474-Pregnancy,
pubmed-meshheading:19909474-Pregnancy Complications,
pubmed-meshheading:19909474-RNA, Viral,
pubmed-meshheading:19909474-Seasons,
pubmed-meshheading:19909474-Sheep
|
| pubmed:year |
2009
|
| pubmed:articleTitle |
Emergence of bluetongue serotypes in Europe, part 2: the occurrence of a BTV-11 strain in Belgium.
|
| pubmed:affiliation |
Section Development of Diagnostic Tools for Epizootic Diseases, Department of Virology, Veterinary and Agrochemical Research Centre, Ukkel, Belgium. krdec@var.fgov.be
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|