| pubmed-article:19908905 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19908905 | lifeskim:mentions | umls-concept:C0013846 | lld:lifeskim |
| pubmed-article:19908905 | lifeskim:mentions | umls-concept:C0030016 | lld:lifeskim |
| pubmed-article:19908905 | lifeskim:mentions | umls-concept:C0439851 | lld:lifeskim |
| pubmed-article:19908905 | lifeskim:mentions | umls-concept:C0600364 | lld:lifeskim |
| pubmed-article:19908905 | lifeskim:mentions | umls-concept:C1552596 | lld:lifeskim |
| pubmed-article:19908905 | lifeskim:mentions | umls-concept:C1705938 | lld:lifeskim |
| pubmed-article:19908905 | lifeskim:mentions | umls-concept:C1947931 | lld:lifeskim |
| pubmed-article:19908905 | lifeskim:mentions | umls-concept:C1527178 | lld:lifeskim |
| pubmed-article:19908905 | pubmed:issue | 22 | lld:pubmed |
| pubmed-article:19908905 | pubmed:dateCreated | 2009-11-13 | lld:pubmed |
| pubmed-article:19908905 | pubmed:abstractText | This paper describes a batch-type coulometric d-fructose biosensor based on direct electron transfer reaction of d-fructose dehydrogenase (FDH) adsorbed on a porous carbon electrode surface. The adsorbed-FDH electrodes catalyzed the electrochemical two-electron oxidation of d-fructose to 5-keto-d-fructose without a mediator. Nanostructured carbon particle-modified electrodes were used for the coulometric d-fructose biosensor to enhance the catalytic current density. The electric charge for the d-fructose oxidation gained by the biocoulometric measurement was in good agreement with the theoretical value corresponding to d-fructose amount in the range from 1 to 100 mM with a sample volume of 1 muL. This method is also applicable to the determination of several oligo/polysaccharides containing the d-fructose unit, in combination with specific hydrolases to yield d-fructose. An example was demonstrated by sucrose determination in which the electrode modified with FDH and invertase was used as a working electrode. To address the problem of electroactive interferences such as ascorbate, the electric charge at the FDH-free electrode was subtracted from the total charge obtained at the FDH-adsorbed electrode. The d-fructose concentrations in several beverages were successfully determined with this method. | lld:pubmed |
| pubmed-article:19908905 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19908905 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19908905 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19908905 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19908905 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19908905 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19908905 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19908905 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:19908905 | pubmed:issn | 1520-6882 | lld:pubmed |
| pubmed-article:19908905 | pubmed:author | pubmed-author:KanoKenjiK | lld:pubmed |
| pubmed-article:19908905 | pubmed:author | pubmed-author:TsujimuraSeiy... | lld:pubmed |
| pubmed-article:19908905 | pubmed:author | pubmed-author:KamitakaYujiY | lld:pubmed |
| pubmed-article:19908905 | pubmed:author | pubmed-author:NishinaAkikoA | lld:pubmed |
| pubmed-article:19908905 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19908905 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:19908905 | pubmed:volume | 81 | lld:pubmed |
| pubmed-article:19908905 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19908905 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19908905 | pubmed:pagination | 9383-7 | lld:pubmed |
| pubmed-article:19908905 | pubmed:meshHeading | pubmed-meshheading:19908905... | lld:pubmed |
| pubmed-article:19908905 | pubmed:meshHeading | pubmed-meshheading:19908905... | lld:pubmed |
| pubmed-article:19908905 | pubmed:meshHeading | pubmed-meshheading:19908905... | lld:pubmed |
| pubmed-article:19908905 | pubmed:meshHeading | pubmed-meshheading:19908905... | lld:pubmed |
| pubmed-article:19908905 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19908905 | pubmed:articleTitle | Coulometric D-fructose biosensor based on direct electron transfer using D-fructose dehydrogenase. | lld:pubmed |
| pubmed-article:19908905 | pubmed:affiliation | Division of Applied Life Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. seiya@kais.kyoto-u.ac.jp | lld:pubmed |
| pubmed-article:19908905 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19908905 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
