Linked Life Data

19908905

Source:http://linkedlifedata.com/resource/pubmed/id/19908905

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2009-11-13
pubmed:abstractText
This paper describes a batch-type coulometric d-fructose biosensor based on direct electron transfer reaction of d-fructose dehydrogenase (FDH) adsorbed on a porous carbon electrode surface. The adsorbed-FDH electrodes catalyzed the electrochemical two-electron oxidation of d-fructose to 5-keto-d-fructose without a mediator. Nanostructured carbon particle-modified electrodes were used for the coulometric d-fructose biosensor to enhance the catalytic current density. The electric charge for the d-fructose oxidation gained by the biocoulometric measurement was in good agreement with the theoretical value corresponding to d-fructose amount in the range from 1 to 100 mM with a sample volume of 1 muL. This method is also applicable to the determination of several oligo/polysaccharides containing the d-fructose unit, in combination with specific hydrolases to yield d-fructose. An example was demonstrated by sucrose determination in which the electrode modified with FDH and invertase was used as a working electrode. To address the problem of electroactive interferences such as ascorbate, the electric charge at the FDH-free electrode was subtracted from the total charge obtained at the FDH-adsorbed electrode. The d-fructose concentrations in several beverages were successfully determined with this method.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1520-6882
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9383-7
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Coulometric D-fructose biosensor based on direct electron transfer using D-fructose dehydrogenase.
pubmed:affiliation
Division of Applied Life Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. seiya@kais.kyoto-u.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't