Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-4-13
pubmed:abstractText
Nonviral integrating vectors can be used for expression of therapeutic genes. piggyBac (PB), a transposon/transposase system, has been used to efficiently generate induced pluripotent stems cells from somatic cells, without genetic alteration. In this paper, we apply PB transposition to express a chimeric antigen receptor (CAR) in primary human T cells. We demonstrate that T cells electroporated to introduce the PB transposon and transposase stably express CD19-specific CAR and when cultured on CD19(+) artificial antigen-presenting cells, numerically expand in a CAR-dependent manner, display a phenotype associated with both memory and effector T cell populations, and exhibit CD19-dependent killing of tumor targets. Integration of the PB transposon expressing CAR was not associated with genotoxicity, based on chromosome analysis. PB transposition for generating human T cells with redirected specificity to a desired target such as CD19 is a new genetic approach with therapeutic implications.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-10411927, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-10537110, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-11683259, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-12393484, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-12649166, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-12955498, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-14583676, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-15170741, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-15385941, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-15583014, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-15663772, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-15780993, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-16096065, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-16177116, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-16269201, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-16352804, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-16607546, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-16877528, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-17005721, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-17039465, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-17164785, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-17299405, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-17309825, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-17339188, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-17576687, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-17635643, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-17932806, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-18060041, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-18227839, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-18354502, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-18369450, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-18413766, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-18413768, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-18679620, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-18725873, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-18768895, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-19224983, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-19252478, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-19265475, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-19414858, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-19752750, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-2549707, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-7645244, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-8673264, http://linkedlifedata.com/resource/pubmed/commentcorrection/19905893-9323364
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1557-7422
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
427-37
pubmed:dateRevised
2011-7-27
pubmed:meshHeading
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