Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3 Pt 1
pubmed:dateCreated
2009-11-12
pubmed:abstractText
We describe the use of a bright-field microscope for dynamic light scattering experiments on weakly scattering samples. The method is based on collecting a time sequence of microscope images and analyzing them in the Fourier space to extract the characteristic time constants as a function of the scattering wave vector. We derive a theoretical model for microscope imaging that accounts for (a) the three-dimensional nature of the sample, (b) the arbitrary coherence properties of the light source, and (c) the effect of the finite numerical aperture of the microscope objective. The model is tested successfully against experiments performed on a colloidal dispersion of small spheres in water, by means of the recently introduced differential dynamic microscopy technique [R. Cerbino and V. Trappe, Phys. Rev. Lett. 100, 188102 (2008)]. Finally, we extend our model to the class of microscopy techniques that can be described by a linear space-invariant imaging of the density of the scattering centers, which includes, for example, dynamic fluorescence microscopy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1550-2376
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
031403
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Scattering information obtained by optical microscopy: differential dynamic microscopy and beyond.
pubmed:affiliation
Dipartimento di Chimica, Biochimica a Biotecnologie per la Medicina, Università degli Studi di Milano, via Fratelli Cervi 93, Segrate, Milano I-20090, Italy. fabio.giavazzi@unimi.it
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't