Linked Life Data

19903484

Source:http://linkedlifedata.com/resource/pubmed/id/19903484

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2010-1-19
pubmed:abstractText
Animal sera provide a non-defined source of nutrients and growth factors for mammalian cell culture. Animal serum supplementation may also introduce experimental artefacts, including immune responses against foreign serum proteins. This artefact is particularly apparent in tumour immunotherapy experiments using dendritic cells (DC) and melanoma cells cultured in fetal calf serum (FCS)-replete media. FCS culture of both DC and melanoma cells significantly enhanced anti-tumour responses in mice immunized with DC that had not been pulsed with tumour antigen. Although serum-free media (SFM) may be used for short term culture of cells, most SFM do not support long term culture of tumour cell lines. In addition, in vivo propagation and re-isolation of tumour cells from rodents is expensive, time consuming and only low numbers of viable tumour cells can be recovered from solid tumours. We show that a defined SFM medium is ideal for routine culture of B16 for use in prophylactic DC immunizations, negating the need for in vivo propagation of tumours to avoid FCS effects in tumour implantation experiments.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1872-7905
pubmed:author
pubmed:copyrightInfo
Copyright 2009 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
31
pubmed:volume
352
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
178-81
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
A defined serum-free medium useful for monitoring anti-melanoma responses induced by dendritic cell immunotherapy.
pubmed:affiliation
Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't