|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1991-3-4
|
|
pubmed:abstractText |
Raf-1 serine- and threonine-specific protein kinase is transiently activated in cells expressing the epidermal growth factor (EGF) receptor upon treatment with EGF. The stimulated EGF receptor coimmunoprecipitates with Raf-1 kinase and mediates protein kinase C-independent phosphorylation of Raf-1 on serine residues. Hyperphosphorylated Raf-1 has lower mobility on sodium dodecyl sulfate gels and has sixfold-increased activity in immunocomplex kinase assay with histone H1 or Raf-1 sequence-derived peptide as a substrate. Raf-1 activation requires kinase-active EGF receptor; a point mutant lacking tyrosine kinase activity in inactive in Raf-1 coupling and association. It is noteworthy that tyrosine phosphorylation of c-Raf-1 induced by EGF was not detected in these cells. These observations suggest that Raf-1 kinase may act as an important downstream effector of EGF signal transduction.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-1690378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-1698619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-1700980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2153914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2157161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2158859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2170413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2188091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2192265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2197270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2197271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-221835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2344614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2466293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2472218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2472219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2475255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2501665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2550926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2556385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2824188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2842341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2881559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2910854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2938016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-2978288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-3003687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-3014651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-3017977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-3043188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-3057494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-3081899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-3257566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-3277669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-3499230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-3501826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-3892533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-3941081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-6196603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-6246487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1990291-942051
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0270-7306
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
11
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
913-9
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:1990291-Amino Acid Sequence,
pubmed-meshheading:1990291-Amino Acids,
pubmed-meshheading:1990291-Animals,
pubmed-meshheading:1990291-Antigen-Antibody Complex,
pubmed-meshheading:1990291-Cell Line,
pubmed-meshheading:1990291-Epidermal Growth Factor,
pubmed-meshheading:1990291-Immune Sera,
pubmed-meshheading:1990291-Kinetics,
pubmed-meshheading:1990291-Mice,
pubmed-meshheading:1990291-Molecular Sequence Data,
pubmed-meshheading:1990291-Phosphorylation,
pubmed-meshheading:1990291-Protein Kinases,
pubmed-meshheading:1990291-Proto-Oncogene Proteins,
pubmed-meshheading:1990291-Proto-Oncogene Proteins c-raf,
pubmed-meshheading:1990291-Receptor, Epidermal Growth Factor,
pubmed-meshheading:1990291-Transfection
|
|
pubmed:year |
1991
|
|
pubmed:articleTitle |
Epidermal growth factor (EGF) stimulates association and kinase activity of Raf-1 with the EGF receptor.
|
|
pubmed:affiliation |
Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick, Maryland 21702.
|
|
pubmed:publicationType |
Journal Article
|
