Source:http://linkedlifedata.com/resource/pubmed/id/19900407
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2010-1-27
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| pubmed:abstractText |
Parkinson's disease is characterized by selective loss of dopaminergic neurons in the substantia nigra and by the appearance of Lewy bodies. Fibrillar alpha-synuclein is the main component of Lewy bodies. Previous studies have suggested that dopamine promotes alpha-synuclein oligomerization and that partially aggregated or oligomeric alpha-synuclein could be cytotoxic. To confirm this hypothesis using cell cultures, we performed size exclusion chromatography as a pretreatment method prior to Western blotting to more clearly detect a small amount of alpha-synuclein oligomers in wild-type alpha-synuclein-overexpressing SH-SY5Y cells. Using this method, we confirmed that stable overexpression of alpha-synuclein in SH-SY5Y cells indeed increased the amounts of alpha-synuclein oligomers in these cells and exposure of the cells to dopamine for 6h facilitated alpha-synuclein oligomerization. These dopamine-induced alpha-synuclein oligomers continued to exist for the following 24h. However, the dopamine-treated cells did not undergo cell death or apoptosis in spite of the presence of increased oligomeric alpha-synuclein. Our data may contribute to the understanding of the mechanisms underlying alpha-synuclein oligomer formation and its suspected cytotoxicity toward dopaminergic neurons.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
1090-2104
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2009 Elsevier Inc. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:day |
1
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| pubmed:volume |
391
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
129-34
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| pubmed:meshHeading |
pubmed-meshheading:19900407-Apoptosis,
pubmed-meshheading:19900407-Blotting, Western,
pubmed-meshheading:19900407-Cell Line, Tumor,
pubmed-meshheading:19900407-Chromatography, Gel,
pubmed-meshheading:19900407-Dopamine,
pubmed-meshheading:19900407-Humans,
pubmed-meshheading:19900407-Lewy Bodies,
pubmed-meshheading:19900407-Neurons,
pubmed-meshheading:19900407-Parkinson Disease,
pubmed-meshheading:19900407-Substantia Nigra,
pubmed-meshheading:19900407-alpha-Synuclein
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| pubmed:year |
2010
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| pubmed:articleTitle |
Dopamine facilitates alpha-synuclein oligomerization in human neuroblastoma SH-SY5Y cells.
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| pubmed:affiliation |
Clinical Research Center, Utano National Hospital, National Hospital Organization, Kyoto 616-8255, Japan.
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| pubmed:publicationType |
Journal Article
|