Linked Life Data

19899955

Source:http://linkedlifedata.com/resource/pubmed/id/19899955

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-4-13
pubmed:abstractText
Polyamidoamine (PAMAM) dendrimer and Tat peptides were conjugated to bacterial magnetic nanoparticles (BMPs) for the construction of an efficient and targeted gene delivery system with transmembrane ability for the gene therapy of brain tumors. Tat-BMPs-PAMAM was complexed with small interfering RNA expression plasmid (psiRNA) corresponding to the open reading frame of the human epidermal growth factor receptor gene (psiRNA-EGFR) to downregulate the EGFR gene by electrostatic interaction. The antitumor effect of psiRNA-EGFR delivered via Tat-BMPs-PAMAM was assessed both in human glioblastoma U251-MG cells and in nude mouse models. Compared with control groups, Tat-BMPs-PAMAM/psiRNA-EGFR resulted in better suppression of EGFR expression and a more obviously arrested effect on the proliferation and invasion ability of U251 cells in vitro. In addition, the growth rate of tumor in the U251 subcutaneous nude mouse model treated with Tat-BMPs-PAMAM/psiRNA-EGFR was slower than in those treated with phosphate-buffered saline or Lipofectamine 2000/psiRNA-Scr. Also, compared with control groups, the expression of oncoproteins (EGFR, p-AKT, MMP2/9, PCNA, VEGF, Bcl-2, and cyclin D1) was obviously downregulated and the number of apoptotic cells was clearly increased in the Tat-BMPs-PAMAM/psiRNA-EGFR treatment groups. In addition, there was no significant difference between the results in vitro and in vivo for the Tat-BMPs-PAMAM/psiRNA-EGFR treatment groups and those of the Lipofectamine 2000/psiRNA-EGFR treatment groups. These results show that Tat-BMPs-PAMAM, with its targeted delivery and transmembrane ability, may be a novel gene delivery system with potential applications in the targeted gene therapy of brain tumors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1557-7422
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
417-26
pubmed:meshHeading
pubmed-meshheading:19899955-Animals, pubmed-meshheading:19899955-Brain Neoplasms, pubmed-meshheading:19899955-Dendrimers, pubmed-meshheading:19899955-Drug Delivery Systems, pubmed-meshheading:19899955-Gene Therapy, pubmed-meshheading:19899955-Glioma, pubmed-meshheading:19899955-Humans, pubmed-meshheading:19899955-Lipids, pubmed-meshheading:19899955-Metal Nanoparticles, pubmed-meshheading:19899955-Mice, pubmed-meshheading:19899955-Mice, Nude, pubmed-meshheading:19899955-Peptide Fragments, pubmed-meshheading:19899955-RNA, Small Interfering, pubmed-meshheading:19899955-RNA Interference, pubmed-meshheading:19899955-Receptor, Epidermal Growth Factor, pubmed-meshheading:19899955-Treatment Outcome, pubmed-meshheading:19899955-Xenograft Model Antitumor Assays, pubmed-meshheading:19899955-tat Gene Products, Human Immunodeficiency Virus
pubmed:year
2010
pubmed:articleTitle
Tat-BMPs-PAMAM conjugates enhance therapeutic effect of small interference RNA on U251 glioma cells in vitro and in vivo.
pubmed:affiliation
Institute of Nanobiotechnology, School of Materials Science and Engineering, Tianjin University, and Tianjin Key Laboratory of Composites and Functional Materials, Tianjin, 300072, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't