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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2009-12-23
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pubmed:abstractText |
D-galacturonic acid can be obtained by hydrolyzing pectin, which is an abundant and low value raw material. By means of metabolic engineering, we constructed fungal strains for the conversion of D-galacturonate to meso-galactarate (mucate). Galactarate has applications in food, cosmetics, and pharmaceuticals and as a platform chemical. In fungi D-galacturonate is catabolized through a reductive pathway with a D-galacturonate reductase as the first enzyme. Deleting the corresponding gene in the fungi Hypocrea jecorina and Aspergillus niger resulted in strains unable to grow on D-galacturonate. The genes of the pathway for D-galacturonate catabolism were upregulated in the presence of D-galacturonate in A. niger, even when the gene for D-galacturonate reductase was deleted, indicating that D-galacturonate itself is an inducer for the pathway. A bacterial gene coding for a D-galacturonate dehydrogenase catalyzing the NAD-dependent oxidation of D-galacturonate to galactarate was introduced to both strains with disrupted D-galacturonate catabolism. Both strains converted D-galacturonate to galactarate. The resulting H. jecorina strain produced galactarate at high yield. The A. niger strain regained the ability to grow on d-galacturonate when the D-galacturonate dehydrogenase was introduced, suggesting that it has a pathway for galactarate catabolism.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19897761-12387863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19897761-16101307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19897761-16879654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19897761-16930134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19897761-17609199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19897761-18759742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19897761-18768163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19897761-19060141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19897761-19159926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19897761-19921179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19897761-2471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19897761-4148097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19897761-4313130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19897761-4392089
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1098-5336
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
76
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
169-75
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pubmed:dateRevised |
2010-9-28
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pubmed:meshHeading |
pubmed-meshheading:19897761-Aspergillus niger,
pubmed-meshheading:19897761-Bacterial Proteins,
pubmed-meshheading:19897761-Biotransformation,
pubmed-meshheading:19897761-Gene Deletion,
pubmed-meshheading:19897761-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:19897761-Gene Expression Regulation, Fungal,
pubmed-meshheading:19897761-Genetic Engineering,
pubmed-meshheading:19897761-Hexuronic Acids,
pubmed-meshheading:19897761-Hypocrea,
pubmed-meshheading:19897761-Metabolic Networks and Pathways,
pubmed-meshheading:19897761-Recombination, Genetic,
pubmed-meshheading:19897761-Sugar Acids
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pubmed:year |
2010
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pubmed:articleTitle |
Metabolic engineering of fungal strains for conversion of D-galacturonate to meso-galactarate.
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pubmed:affiliation |
VTT Technical Research Centre of Finland, Tietotie 2, Espoo, P.O. Box 1500, 02044 VTT, Finland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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