Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1991-3-5
pubmed:abstractText
Rat liver polysome N alpha-acetyltransferase has been purified to homogeneity by a four-step procedure that utilizes ammonium sulfate precipitation, gel filtration, hydroxylapatite chromatography, and Mono Q ion exchange chromatography. The enzyme is greatly stabilized by the inclusion of EDTA and 0.01% deoxycholate in the isolation buffers. The purified enzyme has a native molecular weight of 190,000 and a subunit molecular weight of 95,000, suggesting that it is a homodimer. The enzyme shows a pH optimum of 8.0 and is strongly inhibited by Cl-, I-, SCN-, and ClO4- and to a lesser degree by sulfate and acetate. It is unaffected by phosphate, citrate, and F- and by Na+ and K+; NH4+ is partially inhibitory. The enzyme is also sensitive to iodoacetic acid. It is generally more similar to yeast N alpha-acetyltransferase [Lee, F.-J. S., Lin, L.-W., & Smith, J. A. (1988) J. Biol. Chem. 263, 14948-14955] than to the hen oviduct enzyme, which contains a 7S RNA subunit [Kamitani, K., & Sakiyama, F. (1989) J. Biol. Chem. 264, 13194-13198], although the amino acid compositions are quite different.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1010-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:1989673-Acetyltransferases, pubmed-meshheading:1989673-Amino Acids, pubmed-meshheading:1989673-Ammonium Sulfate, pubmed-meshheading:1989673-Animals, pubmed-meshheading:1989673-Chromatography, pubmed-meshheading:1989673-Citrates, pubmed-meshheading:1989673-Hydrogen-Ion Concentration, pubmed-meshheading:1989673-Iodoacetates, pubmed-meshheading:1989673-Iodoacetic Acid, pubmed-meshheading:1989673-Liver, pubmed-meshheading:1989673-Male, pubmed-meshheading:1989673-Molecular Weight, pubmed-meshheading:1989673-Polyribosomes, pubmed-meshheading:1989673-Potassium Chloride, pubmed-meshheading:1989673-Protease Inhibitors, pubmed-meshheading:1989673-Rats, pubmed-meshheading:1989673-Rats, Inbred Strains, pubmed-meshheading:1989673-Sensitivity and Specificity, pubmed-meshheading:1989673-Substrate Specificity
pubmed:year
1991
pubmed:articleTitle
Rat liver polysome N alpha-acetyltransferase: isolation and characterization.
pubmed:affiliation
Department of Biological Chemistry, College of Medicine, University of California, Irvine 92717.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't