Linked Life Data

19895870

Source:http://linkedlifedata.com/resource/pubmed/id/19895870

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-2-1
pubmed:abstractText
Postmitotic neurons were generated from the human NT2 teratocarcinoma cell line in a novel cell aggregate differentiation procedure. The NT2 model neurons express punctate immunoreactivity for synapsin and for cell markers related to GABAergic and glutamatergic neurotransmission. Using the outside-out patch-clamp configuration, we characterized the kinetics of currents elicited by a rapid application of the amino acid neurotransmitters. Moreover, we detected spontaneous postsynaptic currents in glia free cell cultures that may result from the firing activity of glutamatergic and GABAergic NT2 neurons. These cultured spontaneously active networks may be a useful tool to analyze factors that modulate the formation and efficacy of synapses between human neurons.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1872-7972
pubmed:author
pubmed:copyrightInfo
Copyright 2009. Published by Elsevier Ireland Ltd.
pubmed:issnType
Electronic
pubmed:day
14
pubmed:volume
468
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
207-10
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Synaptic currents and transmitter responses in human NT2 neurons differentiated in aggregate culture.
pubmed:affiliation
Div. of Cell Biology, Institute of Physiology, University of Veterinary Medicine Hannover, Hannover, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't