Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2009-12-7
pubmed:abstractText
FXYD3 is a FXYD-containing Na,K-ATPase ion channel regulator first identified as a protein overexpressed in murine breast tumors initiated by oncogenic ras or neu. However, our preliminary study revealed that FXYD3 expression was down-regulated in oncogenic KRAS-transduced airway epithelial cells. This contradiction led us to investigate the role of FXYD3 in carcinogenesis of the lung. FXYD3 mRNA and protein levels were lower in most of the lung cancer cell lines than in either the noncancerous lung tissue or airway epithelial cells. Protein levels were also lower in a considerable proportion of primary lung cancers than in nontumoral airway epithelia; FXYD3 expression levels decreased in parallel with the dedifferentiation process. Also, a somatic point mutation, g55c (D19H), was found in one cell line. Forced expression of the wild-type FXYD3, but not the mutant, restored the well-demarcated distribution of cortical actin in cancer cells that had lost FXYD3 expression, suggesting FXYD3 plays a role in the maintenance of cytoskeletal integrity. However, no association between FXYD3 expression and its promoter's methylation status was observed. Therefore, inactivation of FXYD3 through a gene mutation or unknown mechanism could be one cause of the atypical shapes of cancer cells and play a potential role in the progression of lung cancer.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1525-2191
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
175
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2646-56
pubmed:dateRevised
2011-3-3
pubmed:meshHeading
pubmed-meshheading:19893046-Amino Acid Sequence, pubmed-meshheading:19893046-Blotting, Western, pubmed-meshheading:19893046-Cell Line, Tumor, pubmed-meshheading:19893046-DNA Methylation, pubmed-meshheading:19893046-DNA Mutational Analysis, pubmed-meshheading:19893046-Down-Regulation, pubmed-meshheading:19893046-Gene Expression, pubmed-meshheading:19893046-Gene Expression Regulation, Neoplastic, pubmed-meshheading:19893046-Gene Silencing, pubmed-meshheading:19893046-Humans, pubmed-meshheading:19893046-Immunohistochemistry, pubmed-meshheading:19893046-Lung Neoplasms, pubmed-meshheading:19893046-Membrane Proteins, pubmed-meshheading:19893046-Molecular Sequence Data, pubmed-meshheading:19893046-Neoplasm Proteins, pubmed-meshheading:19893046-Promoter Regions, Genetic, pubmed-meshheading:19893046-RNA, Messenger, pubmed-meshheading:19893046-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2009
pubmed:articleTitle
Down-regulation of FXYD3 expression in human lung cancers: its mechanism and potential role in carcinogenesis.
pubmed:affiliation
Department of Pathology, Yokohama City University Graduate School of Medicine, Kanazawa-Ku, Yokohama, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't