Source:http://linkedlifedata.com/resource/pubmed/id/19893024
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2009-12-7
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| pubmed:abstractText |
In galactosamine (GalN)-induced rat liver injury, hepatic stem/progenitor cells, small hepatocytes (SHs) and oval cells, transiently appear in the initial period of liver regeneration. To clarify the relationship between SHs and oval cells, CD44(+) and Thy1(+) cells were sorted from GalN-treated livers and used as candidates for SHs and oval cells, respectively. Some Thy1(+) cells isolated 3 days after GalN-treatment (GalN-D3) formed CD44(+) cell colonies, but those from GalN-D2 could form few. GeneChip (Affymetrix, Inc, Santa Clara, CA) analysis of the sorted cells and cultured Thy1(+) cells suggested that hepatocytic differentiation progressed in the order Thy1(+) (GalN-D3), Thy1(+) cell colony (Thy1-C), and CD44(+) (GalN-D4) cells. When Thy1(+), Thy1-C, and CD44(+) cells were transplanted into retrorsine/PH rat livers, they could proliferate to form hepatocytic foci. At 30 days after transplantation most cells forming the foci derived from CD44(+) cells possessed C/EBPalpha(+) nuclei, whereas only a few cells derived from Thy1-C showed this positivity. When Thy1(+) (GalN-D3) cells were cultured between collagen gels in medium with hepatocyte growth factor(+)/dexamethasone(-)/dimethyl sulfoxide(-), ducts/cysts consisting of biliary epithelial cells appeared, whereas with CD44(+) and Thy1(+) (GalN-D2) cells they did not. Taken together, these results indicate that the commitment of Thy1(+) cells to differentiate into hepatocytes or biliary epithelial cells may occur between Day 2 and Day 3. Furthermore, some Thy1(+) cells may differentiate into hepatocytes via CD44(+) SHs.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
1525-2191
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
175
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
2362-71
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| pubmed:dateRevised |
2011-3-3
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| pubmed:meshHeading |
pubmed-meshheading:19893024-Animals,
pubmed-meshheading:19893024-Antigens, CD44,
pubmed-meshheading:19893024-Antigens, Thy-1,
pubmed-meshheading:19893024-Biliary Tract,
pubmed-meshheading:19893024-Cell Differentiation,
pubmed-meshheading:19893024-Cell Separation,
pubmed-meshheading:19893024-Drug-Induced Liver Injury,
pubmed-meshheading:19893024-Epithelial Cells,
pubmed-meshheading:19893024-Flow Cytometry,
pubmed-meshheading:19893024-Galactosamine,
pubmed-meshheading:19893024-Hepatocytes,
pubmed-meshheading:19893024-Immunohistochemistry,
pubmed-meshheading:19893024-Liver Regeneration,
pubmed-meshheading:19893024-Male,
pubmed-meshheading:19893024-Microscopy, Electron, Transmission,
pubmed-meshheading:19893024-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:19893024-Rats,
pubmed-meshheading:19893024-Rats, Inbred F344,
pubmed-meshheading:19893024-Stem Cells
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| pubmed:year |
2009
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| pubmed:articleTitle |
Thy1-positive cells have bipotential ability to differentiate into hepatocytes and biliary epithelial cells in galactosamine-induced rat liver regeneration.
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| pubmed:affiliation |
Department of Pathophysiology, Cancer Research Institute, Sapporo Medical University School of Medicine, Chuo-ku, Sapporo, Japan. junko.kon@ucsf.edu
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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