Source:http://linkedlifedata.com/resource/pubmed/id/19891613
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
2009-11-6
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| pubmed:abstractText |
The development of a protective immune response in sheep towards the presence of the larval stage of Lucilia cuprina has not been reported in the field. Upon investigation of the effects of larval excretory/secretory material on ovine T lymphocyte proliferation, we isolated a 56 kDa protein capable of inhibiting lymphocyte proliferation by at least 70%, compared with that in the presence of mitogen alone. This protein inhibited proliferation induced through cross-linking of the T-cell receptor as well as proliferation induced pharmacologically through the stimulation of the protein kinase C (PKC) pathway. The protein, named blowfly larval immunosuppressive protein (BLIP), was shown to bind directly to lymphocytes. Further investigation revealed that the BLIP prevented a proportion of lymphocytes from entering the first division following stimulation, by affecting the early events in lymphocyte activation. Subsequently, the BLIP reduced CD25 expression on T lymphocytes, reduced IL-2 mRNA expression, in addition to IFN-gamma, IL-4, IL-10 and IL-13 mRNA expression. Conversely, TNF-alpha and TGF-beta gene expression was up-regulated in response to the BLIP. These effects suggest suboptimal activation of T lymphocytes in the presence of the BLIP, and we propose that the BLIP presents an effective immune evasion tactic for the larvae of L. cuprina.
|
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
1365-3024
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
31
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
750-65
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| pubmed:meshHeading |
pubmed-meshheading:19891613-Adaptive Immunity,
pubmed-meshheading:19891613-Amino Acid Sequence,
pubmed-meshheading:19891613-Animals,
pubmed-meshheading:19891613-Cell Proliferation,
pubmed-meshheading:19891613-Cytokines,
pubmed-meshheading:19891613-Diptera,
pubmed-meshheading:19891613-Gene Expression Regulation,
pubmed-meshheading:19891613-Insect Proteins,
pubmed-meshheading:19891613-Interleukin-2 Receptor alpha Subunit,
pubmed-meshheading:19891613-Larva,
pubmed-meshheading:19891613-Lymphocyte Activation,
pubmed-meshheading:19891613-Molecular Sequence Data,
pubmed-meshheading:19891613-Myiasis,
pubmed-meshheading:19891613-Protein Binding,
pubmed-meshheading:19891613-Sequence Alignment,
pubmed-meshheading:19891613-Sheep,
pubmed-meshheading:19891613-Sheep Diseases,
pubmed-meshheading:19891613-T-Lymphocytes
|
| pubmed:year |
2009
|
| pubmed:articleTitle |
A Lucilia cuprina excretory-secretory protein inhibits the early phase of lymphocyte activation and subsequent proliferation.
|
| pubmed:affiliation |
Queensland Agricultural Biotechnology Centre, University of Queensland, Brisbane, Qld, Australia. rebecca.elkington@dpi.qld.gov.au
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|