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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2010-1-21
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pubmed:abstractText |
In this study, we searched for anther-specific genes involved in male gametophyte development in apple (Malus x domestica Borkh. cv. Fuji) by differential display-PCR. Three full-length cDNAs were isolated, and the corresponding genomic sequences were determined by genome walking. The identified genes showed intronless 228- to 264-bp open reading frames and shared 82-90% nucleotide sequence. Sequence analysis identified that they encoded a putative arabinogalactan protein (AGP) and were designated MdAGP1, MdAGP2, and MdAGP3, respectively. RT (reverse transcriptase)-PCR revealed that the MdAGP genes were selectively expressed in the stamen. Promoter analysis confirmed that the MdAGP3 promoter was capable of directing anther- or pollen-specific expression of the GUS reporter in tobacco and apple. Furthermore, expression of ribosome-inactivating protein under the control of the MdAGP3 promoter induced complete sporophytic male sterility as we had expected.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1432-203X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
15-24
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pubmed:meshHeading |
pubmed-meshheading:19890636-Amino Acid Sequence,
pubmed-meshheading:19890636-DNA, Complementary,
pubmed-meshheading:19890636-DNA, Plant,
pubmed-meshheading:19890636-Flowers,
pubmed-meshheading:19890636-Gene Expression Profiling,
pubmed-meshheading:19890636-Gene Expression Regulation, Plant,
pubmed-meshheading:19890636-Gene Library,
pubmed-meshheading:19890636-Genes, Plant,
pubmed-meshheading:19890636-Genome, Plant,
pubmed-meshheading:19890636-Malus,
pubmed-meshheading:19890636-Molecular Sequence Data,
pubmed-meshheading:19890636-Mucoproteins,
pubmed-meshheading:19890636-Plant Infertility,
pubmed-meshheading:19890636-Plant Proteins,
pubmed-meshheading:19890636-Plants, Genetically Modified,
pubmed-meshheading:19890636-Promoter Regions, Genetic,
pubmed-meshheading:19890636-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19890636-Sequence Alignment,
pubmed-meshheading:19890636-Sequence Analysis, DNA,
pubmed-meshheading:19890636-Tobacco
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pubmed:year |
2010
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pubmed:articleTitle |
Isolation and promoter analysis of anther-specific genes encoding putative arabinogalactan proteins in Malus x domestica.
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pubmed:affiliation |
Biotech Research Team, Dongbu Advanced Research Institute, Dongbu HiTek Co., Ltd, Daejeon, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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