Source:http://linkedlifedata.com/resource/pubmed/id/19889024
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2010-1-27
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| pubmed:abstractText |
Dendritic cells (DC) are potent antigen-presenting cells capable to induce efficient antigen-specific T cell responses in vitro and in vivo. Herein, the maturation process is of great significance, as immature DC (iDC) are known to induce rather regulatory than effector T cell differentiation. This study was designed to characterize the role of the CD40-CD40L pathway for differentiation and function of human DC. Therefore, iDC were stimulated through CD40-CD40L interaction by transduction of DC with adenoviral vectors encoding for CD40L (Ad-CD40L). Resulting DC (CD40L-DC) were analysed concerning their phenotype, cytokine profile and T cell stimulatory capacity. Transduction induced a DC phenotype comparable to stimulation with proinflammatory cytokines as revealed by upregulation of CD83 and the costimulatory molecules CD80 and CD86. Additionally, Ad-CD40L-induced strong production of IL-12p70 not observed in cytokine-matured DC. Surprisingly, the T cell stimulatory capacity was markedly reduced in CD40L-DC. Furthermore, stimulation of CD8(+) T cells by peptide-loaded CD40L-DC resulted in a substantial reduction of antigen-specific IFN-gamma production. This phenomenon is due to an enhanced IL-10 production of CD40L-DC in DC-T cell coculture as well as a stabilization of the IL-10 receptor expression on activated T cells. These data demonstrate that DC stimulated through CD40-CD40L interaction differentiate into tolerogenic DC with immunomodulatory function.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/CD40 Ligand,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-10
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
1600-0625
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
19
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
44-53
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| pubmed:meshHeading |
pubmed-meshheading:19889024-Adenoviridae,
pubmed-meshheading:19889024-Antigens, CD40,
pubmed-meshheading:19889024-Autocrine Communication,
pubmed-meshheading:19889024-CD4-Positive T-Lymphocytes,
pubmed-meshheading:19889024-CD40 Ligand,
pubmed-meshheading:19889024-CD8-Positive T-Lymphocytes,
pubmed-meshheading:19889024-Cell Proliferation,
pubmed-meshheading:19889024-Cells, Cultured,
pubmed-meshheading:19889024-Cytokines,
pubmed-meshheading:19889024-Dendritic Cells,
pubmed-meshheading:19889024-Gene Transfer Techniques,
pubmed-meshheading:19889024-Genetic Vectors,
pubmed-meshheading:19889024-Humans,
pubmed-meshheading:19889024-Immunomodulation,
pubmed-meshheading:19889024-Interleukin-10,
pubmed-meshheading:19889024-Phenotype,
pubmed-meshheading:19889024-Receptors, Interleukin-10,
pubmed-meshheading:19889024-Signal Transduction
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| pubmed:year |
2010
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| pubmed:articleTitle |
CD40 signalling induces IL-10-producing, tolerogenic dendritic cells.
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| pubmed:affiliation |
Department of Dermatology, Johannes-Gutenberg-University, Mainz, Germany. tuettenberg@hautklinik.klinik.uni-mainz.de
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|