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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1991-2-27
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pubmed:abstractText |
The influence of native (N-) and oxidized (Ox-) low density lipoproteins (LDLs) on endothelium-dependent vasomotion is still controversial. We investigated the short-term effects of N-LDL and Ox-LDL on the formation of endothelium-derived relaxing factor (EDRF) in native and cultured endothelial cells and on its inactivation after release from the cells. N-LDL was isolated from fresh human plasma via sequential ultracentrifugation and oxidized by incubation with Cu2+. EDRF released from cultured endothelial cells was inactivated by both N-LDL and Ox-LDL (1 mg/ml) as detected in a bioassay system. N-LDL reduced the EDRF-mediated vasodilations of the detector segments by 38.5 +/- 5.3%, and Ox-LDL, by 55.5 +/- 4.6%. The effects of lipoproteins on EDRF formation were studied in cultured endothelial cells preincubated with either N-LDL or Ox-LDL (1 mg/ml for 1 hour) and stimulated for EDRF release with bradykinin after washout of the lipoproteins. EDRF was assessed by measuring its stimulatory effect on the activity of a purified, soluble guanylate cyclase. Both N-LDL and Ox-LDL did not reduce the bradykinin-induced EDRF formation. Consistent with this finding, acetylcholine-induced, EDRF-mediated dilations of intact rabbit femoral artery segments were not impaired by luminal exposure to N-LDL or Ox-LDL (1 mg/ml for 1 hour). However, these relaxations were significantly reduced by preincubation of aortic ring preparations with the same concentrations of the same charges of N-LDL and Ox-LDL. In conclusion, neither N-LDL nor Ox-LDL acutely impairs the formation of EDRF but does inactivate EDRF after its release from endothelial cells.(ABSTRACT TRUNCATED AT 250 WORDS)
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
1049-8834
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
198-203
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1987998-Acetylcholine,
pubmed-meshheading:1987998-Animals,
pubmed-meshheading:1987998-Aorta,
pubmed-meshheading:1987998-Cells, Cultured,
pubmed-meshheading:1987998-Dose-Response Relationship, Drug,
pubmed-meshheading:1987998-Endothelium, Vascular,
pubmed-meshheading:1987998-Female,
pubmed-meshheading:1987998-Femoral Artery,
pubmed-meshheading:1987998-Humans,
pubmed-meshheading:1987998-Lipoproteins, LDL,
pubmed-meshheading:1987998-Male,
pubmed-meshheading:1987998-Nitric Oxide,
pubmed-meshheading:1987998-Oxidation-Reduction,
pubmed-meshheading:1987998-Rabbits,
pubmed-meshheading:1987998-Vasodilation
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pubmed:articleTitle |
Effects of native and oxidized low density lipoproteins on formation and inactivation of endothelium-derived relaxing factor.
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pubmed:affiliation |
Institute of Applied Physiology, University of Freiburg, F.R.G.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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