Linked Life Data

19879857

Source:http://linkedlifedata.com/resource/pubmed/id/19879857

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2010-1-27
pubmed:abstractText
YC-1 has recently been demonstrated to have potent anti-invasion and anti-metastatic activity in several cancer models, in addition to its anti-proliferation activity. However, the mechanism underlying its anti-invasion/anti-metastatic activity is largely unknown. Nasopharyngeal carcinoma (NPC) is a highly metastatic head and neck cancer in Southeast Asia. Here, we demonstrated that YC-1 inhibited invasiveness and proliferation of NPC cells, with the latter being accompanied by PARP cleavage, S-phase arrest and activation of Chk1/Chk2. We aimed at identifying novel anti-invasion mechanisms of YC-1 in NPC by a functional proteomic platform, the reverse phase protein array (RPPA). Our study revealed for the first time that multiple invasion-related signaling proteins (beta-catenin, caveolin, Src and EGFR), as well as several growth-related proteins (AMPKalpha, phospho-acetyl-CoA carboxylase (p-ACC), HER-2 and mTOR), which were previously un-described signaling proteins altered by YC-1, were found to be down-modulated by YC-1 in NPC cells. We hypothesized that YC-1-mediated downregulation of these invasion proteins contributed to its anti-invasion activity in NPC cells. Overexpression of EGFR, activated Src or caveolin, but not beta-catenin reversed the inhibitory effects of YC-1 on NPC cell invasion, with EGFR and activated Src having additional effects on rescuing NPC cells from YC-1-mediated growth inhibition. In summary, we have identified several novel anti-invasion mechanisms of YC-1 that could impact NPC, and possibly other cancers as well.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1873-2968
pubmed:author
pubmed:copyrightInfo
Copyright 2009. Published by Elsevier Inc.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
842-52
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:19879857-Antineoplastic Agents, pubmed-meshheading:19879857-Carcinoma, pubmed-meshheading:19879857-Cell Line, Tumor, pubmed-meshheading:19879857-Cell Proliferation, pubmed-meshheading:19879857-Down-Regulation, pubmed-meshheading:19879857-Furans, pubmed-meshheading:19879857-Gene Expression Regulation, Neoplastic, pubmed-meshheading:19879857-Humans, pubmed-meshheading:19879857-Indazoles, pubmed-meshheading:19879857-Nasopharyngeal Neoplasms, pubmed-meshheading:19879857-Neoplasm Invasiveness, pubmed-meshheading:19879857-Neoplasm Proteins, pubmed-meshheading:19879857-Poly(ADP-ribose) Polymerases, pubmed-meshheading:19879857-Protein Array Analysis, pubmed-meshheading:19879857-Protein Kinases, pubmed-meshheading:19879857-Protein-Serine-Threonine Kinases, pubmed-meshheading:19879857-S Phase
pubmed:year
2010
pubmed:articleTitle
Reverse phase protein array identifies novel anti-invasion mechanisms of YC-1.
pubmed:affiliation
Cancer Signaling Laboratory, Department of Clinical Oncology, The Chinese University of Hong Kong, Hong Kong.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't