Linked Life Data

19878643

Source:http://linkedlifedata.com/resource/pubmed/id/19878643

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-1-27
pubmed:abstractText
Lewis histo-blood group antigens are one of the major classes of biologically active oligosaccharides. In this work, underivatized Lewis blood groups were studied by electrospray tandem mass spectrometry (ESI-MS(n)) in the positive mode with three different mass analyzers: Q-TOF (quadrupole time-of-flight), QqQ (triple quadrupole), and LIT (linear ion trap). It was observed that, under collision-induced fragmentations, type 1 Lewis antigens (Le(a) and Le(b)) could be distinguished from type 2 (Le(x) and Le(y)) on the basis of specific fragmentations of the GlcNAc unit. Whereas O-4-linked sugars of the GlcNAc are lost as residues, the O-3-linked sugars undergo fragmentation both as sugar units and as sugar residues (unit -18Da). Type 2 Lewis antigens also showed a characteristic cross-ring cleavage (0,2)A(2) of the GlcNAc. As a result, the product ions at m/z 388 and 305, characteristic of Le(x), and m/z 372, characteristic of Le(a), are proposed to distinguish the trisaccharide isomers Le(x)/Le(a). Also, the product ions at m/z 534 and 305, characteristic of Le(y), and m/z 372, characteristic of Le(b), are proposed to distinguish the tetrasaccharide isomers Le(b)/Le(y). These diagnostic fragment ions were further applied in the identification of Lewis type 2 antigens (Le(x) and Le(y)) in the lipopolysaccharide of the human gastric pathogen, Helicobacter pylori.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1096-0309
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2009 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
397
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
186-96
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Differentiation of isomeric Lewis blood groups by positive ion electrospray tandem mass spectrometry.
pubmed:affiliation
Departamento de Química, Universidade de Aveiro, 3810-193 Aveiro, Portugal.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't