Linked Life Data

19874919

Source:http://linkedlifedata.com/resource/pubmed/id/19874919

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-3-1
pubmed:abstractText
Water-insoluble regenerated silk materials are normally produced by increasing the beta-sheet content (silk II). In the present study water-insoluble silk films were prepared by controlling the very slow drying of Bombyx mori silk solutions, resulting in the formation of stable films with a predominant silk I instead of silk II structure. Wide angle X-ray scattering indicated that the silk films stabilized by slow drying were mainly composed of silk I rather than silk II, while water- and methanol-annealed silk films had a higher silk II content. The silk films prepared by slow drying had a globule-like structure at the core surrounded by nano-filaments. The core region was composed of silk I and silk II, surrounded by hydrophilic nano-filaments containing random turns and alpha-helix secondary structures. The insoluble silk films prepared by slow drying had unique thermal, mechanical and degradative properties. Differential scanning calorimetry results revealed that silk I crystals had stable thermal properties up to 250 degrees C, without crystallization above the T(g), but degraded at lower temperatures than silk II structure. Compared with water- and methanol-annealed films the films prepared by slow drying had better mechanical ductility and were more rapidly enzymatically degraded, reflecting the differences in secondary structure achieved via differences in post processing of the cast silk films. Importantly, the silk I structure, a key intermediate secondary structure for the formation of mechanically robust natural silk fibers, was successfully generated by the present approach of very slow drying, mimicking the natural process. The results also point to a new mode of generating new types of silk biomaterials with enhanced mechanical properties and increased degradation rates, while maintaining water insolubility, along with a low beta-sheet content.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19874919-10777765, http://linkedlifedata.com/resource/pubmed/commentcorrection/19874919-11518580, http://linkedlifedata.com/resource/pubmed/commentcorrection/19874919-12425660, http://linkedlifedata.com/resource/pubmed/commentcorrection/19874919-12944968, http://linkedlifedata.com/resource/pubmed/commentcorrection/19874919-15132651, http://linkedlifedata.com/resource/pubmed/commentcorrection/19874919-15132657, http://linkedlifedata.com/resource/pubmed/commentcorrection/19874919-15132659, http://linkedlifedata.com/resource/pubmed/commentcorrection/19874919-15132662, http://linkedlifedata.com/resource/pubmed/commentcorrection/19874919-15518557, http://linkedlifedata.com/resource/pubmed/commentcorrection/19874919-15585282, http://linkedlifedata.com/resource/pubmed/commentcorrection/19874919-16637042, http://linkedlifedata.com/resource/pubmed/commentcorrection/19874919-16853155, http://linkedlifedata.com/resource/pubmed/commentcorrection/19874919-17218036, http://linkedlifedata.com/resource/pubmed/commentcorrection/19874919-17292467, http://linkedlifedata.com/resource/pubmed/commentcorrection/19874919-17607763, http://linkedlifedata.com/resource/pubmed/commentcorrection/19874919-17997479, http://linkedlifedata.com/resource/pubmed/commentcorrection/19874919-18502501
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1878-7568
pubmed:author
pubmed:copyrightInfo
Copyright 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1380-7
pubmed:dateRevised
2011-8-1
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Water-insoluble silk films with silk I structure.
pubmed:affiliation
Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural