Linked Life Data

19874887

Source:http://linkedlifedata.com/resource/pubmed/id/19874887

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-2-3
pubmed:abstractText
Ultraviolet A (UVA) radiation is an oxidizing agent that strongly induces the heme oxygenase 1 (HO-1) gene and expression of the protein in cultured human skin fibroblasts but weakly induces it in skin keratinocytes. Lower basal levels of HO-1 and much higher basal levels of HO-2 protein are observed in keratinocytes compared with fibroblasts. Using both overexpression and knockdown approaches, we demonstrate that HO-2 modulates basal and UVA-induced HO-1 protein levels, whereas HO-1 levels do not affect HO-2 levels in skin fibroblasts and keratinocytes. Silencing of Bach1 strongly increases HO-1 levels in transformed HaCaT keratinocytes and these HO-1 levels are not further increased by either UVA irradiation or silencing of HO-2. This is consistent with the conclusion that high constitutive levels of HO-2 expression in keratinocytes are responsible for the resistance of these cells to HO-1 induction by UVA radiation and that Bach1 plays a predominant role in influencing the lack of HO-1 expression in keratinocytes. Bach1 inhibition leading to HO-1 induction reduced UVA-irradiation-induced damage as monitored both by the extent of LDH release and by nuclear condensation, so that Bach1 inhibition seems to protect against UVA-irradiation-induced damage in keratinocytes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1873-4596
pubmed:author
pubmed:copyrightInfo
Copyright 2009 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
196-206
pubmed:meshHeading
pubmed-meshheading:19874887-Basic-Leucine Zipper Transcription Factors, pubmed-meshheading:19874887-Fanconi Anemia Complementation Group Proteins, pubmed-meshheading:19874887-Fibroblasts, pubmed-meshheading:19874887-Gene Expression Regulation, Enzymologic, pubmed-meshheading:19874887-HeLa Cells, pubmed-meshheading:19874887-Heme Oxygenase (Decyclizing), pubmed-meshheading:19874887-Heme Oxygenase-1, pubmed-meshheading:19874887-Humans, pubmed-meshheading:19874887-Keratinocytes, pubmed-meshheading:19874887-NF-E2-Related Factor 2, pubmed-meshheading:19874887-Oxidative Stress, pubmed-meshheading:19874887-RNA, Small Interfering, pubmed-meshheading:19874887-Radiation Tolerance, pubmed-meshheading:19874887-Skin, pubmed-meshheading:19874887-Transfection, pubmed-meshheading:19874887-Ultraviolet Rays
pubmed:year
2010
pubmed:articleTitle
A role for Bach1 and HO-2 in suppression of basal and UVA-induced HO-1 expression in human keratinocytes.
pubmed:affiliation
Department of Pharmacy and Pharmacology, University of Bath, Bath BA2 7AY, UK.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't