| pubmed-article:1987380 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1987380 | lifeskim:mentions | umls-concept:C0521026 | lld:lifeskim |
| pubmed-article:1987380 | lifeskim:mentions | umls-concept:C1012334 | lld:lifeskim |
| pubmed-article:1987380 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:1987380 | lifeskim:mentions | umls-concept:C0206256 | lld:lifeskim |
| pubmed-article:1987380 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:1987380 | lifeskim:mentions | umls-concept:C0013879 | lld:lifeskim |
| pubmed-article:1987380 | lifeskim:mentions | umls-concept:C0205374 | lld:lifeskim |
| pubmed-article:1987380 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:1987380 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:1987380 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:1987380 | pubmed:dateCreated | 1991-2-19 | lld:pubmed |
| pubmed-article:1987380 | pubmed:abstractText | Autographa californica nuclear polyhedrosis virus (AcMNPV) is a double-stranded DNA virus that expresses several immediate-early genes under the control of different promoters. The expression of one of these transcription units, IE-N, is shown here, by a transient expression assay, to be regulated by both cis- and trans-acting viral elements. The steady-state levels of IE-N mRNA were very abundant soon after infection but were nearly undetectable during the late phase of the viral life cycle. Analysis of the transient expression of a reporter construct driven by the IE-N promoter (IE-NCAT) was conducted to define viral elements which regulate IE-N gene expression. Viral enhancer hr1 and two immediate-early genes, IE-1 and IE-N, were shown to affect relative levels of reporter enzyme activity produced by IE-NCAT. The hr1 enhancer stimulated the expression of IE-NCAT, independent of orientation and position relative to the promoter and in the absence of any trans-acting viral factors. Regulation of IE-NCAT expression by the IE-1 and IE-N genes required less than 290 bp of promoter sequences upstream of the site of transcription initiation and was not dependent upon the hr1 enhancer. Coexpression of the IE-N gene had an autostimulatory effect upon IE-NCAT activity, whereas coexpression of the IE-1 gene reduced levels of reporter activity. The levels of reporter activity measured upon coexpression of either immediate-early gene with IE-NCAT linked to the hr1 enhancer appear to be the combined result of both cis- and trans-regulatory elements influencing expression from IE-NCAT. These results suggest that IE-N gene expression in baculovirus infection may be influenced by the concerted activity of three AcMNPV regulatory elements. | lld:pubmed |
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| pubmed-article:1987380 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1987380 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1987380 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1987380 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1987380 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:1987380 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:1987380 | pubmed:author | pubmed-author:GuarinoL ALA | lld:pubmed |
| pubmed-article:1987380 | pubmed:author | pubmed-author:CarsonD DDD | lld:pubmed |
| pubmed-article:1987380 | pubmed:author | pubmed-author:SummersM DMD | lld:pubmed |
| pubmed-article:1987380 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1987380 | pubmed:volume | 65 | lld:pubmed |
| pubmed-article:1987380 | pubmed:geneSymbol | IE-N | lld:pubmed |
| pubmed-article:1987380 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1987380 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1987380 | pubmed:pagination | 945-51 | lld:pubmed |
| pubmed-article:1987380 | pubmed:dateRevised | 2010-9-9 | lld:pubmed |
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| pubmed-article:1987380 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1987380 | pubmed:articleTitle | Transient expression of the Autographa californica nuclear polyhedrosis virus immediate-early gene, IE-N, is regulated by three viral elements. | lld:pubmed |
| pubmed-article:1987380 | pubmed:affiliation | Department of Biochemistry, Texas A&M University, College Station 77843. | lld:pubmed |
| pubmed-article:1987380 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1987380 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:1987380 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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