Linked Life Data

1987380

Source:http://linkedlifedata.com/resource/pubmed/id/1987380

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-2-19
pubmed:abstractText
Autographa californica nuclear polyhedrosis virus (AcMNPV) is a double-stranded DNA virus that expresses several immediate-early genes under the control of different promoters. The expression of one of these transcription units, IE-N, is shown here, by a transient expression assay, to be regulated by both cis- and trans-acting viral elements. The steady-state levels of IE-N mRNA were very abundant soon after infection but were nearly undetectable during the late phase of the viral life cycle. Analysis of the transient expression of a reporter construct driven by the IE-N promoter (IE-NCAT) was conducted to define viral elements which regulate IE-N gene expression. Viral enhancer hr1 and two immediate-early genes, IE-1 and IE-N, were shown to affect relative levels of reporter enzyme activity produced by IE-NCAT. The hr1 enhancer stimulated the expression of IE-NCAT, independent of orientation and position relative to the promoter and in the absence of any trans-acting viral factors. Regulation of IE-NCAT expression by the IE-1 and IE-N genes required less than 290 bp of promoter sequences upstream of the site of transcription initiation and was not dependent upon the hr1 enhancer. Coexpression of the IE-N gene had an autostimulatory effect upon IE-NCAT activity, whereas coexpression of the IE-1 gene reduced levels of reporter activity. The levels of reporter activity measured upon coexpression of either immediate-early gene with IE-NCAT linked to the hr1 enhancer appear to be the combined result of both cis- and trans-regulatory elements influencing expression from IE-NCAT. These results suggest that IE-N gene expression in baculovirus infection may be influenced by the concerted activity of three AcMNPV regulatory elements.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1987380-16789237, http://linkedlifedata.com/resource/pubmed/commentcorrection/1987380-16789258, http://linkedlifedata.com/resource/pubmed/commentcorrection/1987380-16789259, http://linkedlifedata.com/resource/pubmed/commentcorrection/1987380-16789264, http://linkedlifedata.com/resource/pubmed/commentcorrection/1987380-2642976, http://linkedlifedata.com/resource/pubmed/commentcorrection/1987380-2826808, http://linkedlifedata.com/resource/pubmed/commentcorrection/1987380-2985470, http://linkedlifedata.com/resource/pubmed/commentcorrection/1987380-3043024, http://linkedlifedata.com/resource/pubmed/commentcorrection/1987380-3277328, http://linkedlifedata.com/resource/pubmed/commentcorrection/1987380-3944847, http://linkedlifedata.com/resource/pubmed/commentcorrection/1987380-518835, http://linkedlifedata.com/resource/pubmed/commentcorrection/1987380-6154876, http://linkedlifedata.com/resource/pubmed/commentcorrection/1987380-6321770, http://linkedlifedata.com/resource/pubmed/commentcorrection/1987380-688389, http://linkedlifedata.com/resource/pubmed/commentcorrection/1987380-6960240
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:geneSymbol
IE-N
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
945-51
pubmed:dateRevised
2010-9-9
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Transient expression of the Autographa californica nuclear polyhedrosis virus immediate-early gene, IE-N, is regulated by three viral elements.
pubmed:affiliation
Department of Biochemistry, Texas A&M University, College Station 77843.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't