Linked Life Data

19864104

Source:http://linkedlifedata.com/resource/pubmed/id/19864104

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-11-9
pubmed:abstractText
A rapid and high sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for simultaneous determination of chlorogenic acid and cinnamic acid in human plasma was developed. The analytes and internal standard (IS), tinidazole, were extracted from human plasma via liquid/liquid extraction with ether-ethyl acetate (1:1, v/v) and separated on an Agilent Zorbax SB C18 column within 5min. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization technique, operating in multiple reaction monitoring (MRM) and negative ion mode. The precursor to product ion transitions monitored for chlorogenic acid, cinnamic acid and IS were m/z 352.9-->191.1, 146.8-->103.1, 245.6-->126.0, respectively. The assay was validated with linear range of 1.00-800.00ng/mL for chlorogenic acid and 0.50-400.00ng/mL for cinnamic acid. The intra- and inter-day precisions (RSD%) were within 9.05% for each analyte. The absolution recoveries were greater than 74.62% for chlorogenic acid and 76.21% for cinnamic acid. Each analyte was proved to be stable during all sample storage, preparation and analytic procedures. The method was successfully applied to a pharmacokinetic study of Mailuoning injection in 10 healthy volunteers.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1873-264X
pubmed:author
pubmed:issnType
Electronic
pubmed:day
5
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
685-90
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Liquid chromatograph/tandem mass spectrometry assay for the simultaneous determination of chlorogenic acid and cinnamic acid in plasma and its application to a pharmacokinetic study.
pubmed:affiliation
Clinic Pharmacology Laboratory, The Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, No. 155 Hanzhong Road, Nanjing, Jiangsu 210029, PR China. jennifer2886@126.com
pubmed:publicationType
Journal Article, Comparative Study