Linked Life Data

1986400

Source:http://linkedlifedata.com/resource/pubmed/id/1986400

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1991-2-14
pubmed:abstractText
A plasma membrane-rich fraction has been separated from liver cells and cells of two solid rat tumors. D23 hepatoma and MC7 sarcoma. On the basis of marker enzyme activity the membranes separating at the 31-41% interface on the discontinuous sucrose gradient were enriched 15- to 19-fold. No significant differences in the phospholipid (PL) composition of the three membrane fractions were observed. The PL fatty acid (FA) composition showed that the percentage of unsaturated FA in all three membranes was between 43 and 48%. However, the oleic acid:PUFA ratio was much greater from tumor membranes. Membrane cholesterol was also significantly lower for cells from both tumors compared with liver cells. The DPH fluorescence polarization of the membrane fractions showed that the membranes from cells of both tumors are significantly less ordered than those of liver at all temperatures measured (4-50 degrees C). The Mg2+ ATPase activity of the plasma membranes is inactivated by hyperthermia treatments. The enzyme from liver cells was more thermostable (LT50 = 53.86 degrees C) than that from cells of either D23 (LT50 = 47.51 degrees C) or MC7 (LT50 = 46.34 degrees C) tumors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0033-7587
pubmed:author
pubmed:issnType
Print
pubmed:volume
125
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
48-55
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Lipid composition of the membranes from cells of two rat tumors and its relationship to tumor thermosensitivity.
pubmed:affiliation
School of Biochemistry, University of Birmingham, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't