pubmed-article:19859540 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:19859540 | lifeskim:mentions | umls-concept:C0032098 | lld:lifeskim |
pubmed-article:19859540 | lifeskim:mentions | umls-concept:C1099354 | lld:lifeskim |
pubmed-article:19859540 | lifeskim:mentions | umls-concept:C0242356 | lld:lifeskim |
pubmed-article:19859540 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
pubmed-article:19859540 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
pubmed-article:19859540 | lifeskim:mentions | umls-concept:C0449445 | lld:lifeskim |
pubmed-article:19859540 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
pubmed-article:19859540 | lifeskim:mentions | umls-concept:C1517004 | lld:lifeskim |
pubmed-article:19859540 | lifeskim:mentions | umls-concept:C1709634 | lld:lifeskim |
pubmed-article:19859540 | pubmed:dateCreated | 2009-10-27 | lld:pubmed |
pubmed-article:19859540 | pubmed:abstractText | TAS loci in plant genomes encode transacting small interfering RNAs (ta-siRNAs) that regulate expression of a number of genes. The function of TAS3 precursor in Arabidopsis thaliana is controlled by two miR390 target sites flanking two ta-siARF sequences targeting mRNAs of ARF transcription factors. Cleavage of the 3'-miR390-site initiates ta-siRNAs biogenesis. Here we describe the new method for identification of plant ta-siRNA precursors based on PCR with oligodeoxyribonucleotide primers mimicking miR390. The method was found to be efficient for dicotiledonous plants, cycads, and mosses. Based on sequences of amplified loci and a database analysis, a novel type of miR390-dependent TAS sequences was identified in dicots. These TAS loci are characterized by a smaller distance between miR390 sites compared to TAS3, a single copy of ta-siARF, and a sequence conservation pattern pointing to the possibility that processing of novel TAS-like locus is initiated by cleavage of the 5'-terminal miR390 target site. | lld:pubmed |
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pubmed-article:19859540 | pubmed:language | eng | lld:pubmed |
pubmed-article:19859540 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19859540 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:19859540 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19859540 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19859540 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19859540 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19859540 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19859540 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19859540 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:19859540 | pubmed:issn | 1110-7251 | lld:pubmed |
pubmed-article:19859540 | pubmed:author | pubmed-author:OzerovaL VLV | lld:pubmed |
pubmed-article:19859540 | pubmed:author | pubmed-author:MorozovS YSY | lld:pubmed |
pubmed-article:19859540 | pubmed:author | pubmed-author:TroitskyA VAV | lld:pubmed |
pubmed-article:19859540 | pubmed:author | pubmed-author:SolovyevA GAG | lld:pubmed |
pubmed-article:19859540 | pubmed:author | pubmed-author:BobrovaV KVK | lld:pubmed |
pubmed-article:19859540 | pubmed:author | pubmed-author:MilyutinaI... | lld:pubmed |
pubmed-article:19859540 | pubmed:author | pubmed-author:KrasnikovaM... | lld:pubmed |
pubmed-article:19859540 | pubmed:issnType | Electronic | lld:pubmed |
pubmed-article:19859540 | pubmed:volume | 2009 | lld:pubmed |
pubmed-article:19859540 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:19859540 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:19859540 | pubmed:pagination | 952304 | lld:pubmed |
pubmed-article:19859540 | pubmed:dateRevised | 2010-9-28 | lld:pubmed |
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pubmed-article:19859540 | pubmed:year | 2009 | lld:pubmed |
pubmed-article:19859540 | pubmed:articleTitle | Novel miR390-dependent transacting siRNA precursors in plants revealed by a PCR-based experimental approach and database analysis. | lld:pubmed |
pubmed-article:19859540 | pubmed:affiliation | Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences, Timiryazevskaya 42, 127550 Moscow, Russia. | lld:pubmed |
pubmed-article:19859540 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:19859540 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |