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pubmed-article:19859540pubmed:abstractTextTAS loci in plant genomes encode transacting small interfering RNAs (ta-siRNAs) that regulate expression of a number of genes. The function of TAS3 precursor in Arabidopsis thaliana is controlled by two miR390 target sites flanking two ta-siARF sequences targeting mRNAs of ARF transcription factors. Cleavage of the 3'-miR390-site initiates ta-siRNAs biogenesis. Here we describe the new method for identification of plant ta-siRNA precursors based on PCR with oligodeoxyribonucleotide primers mimicking miR390. The method was found to be efficient for dicotiledonous plants, cycads, and mosses. Based on sequences of amplified loci and a database analysis, a novel type of miR390-dependent TAS sequences was identified in dicots. These TAS loci are characterized by a smaller distance between miR390 sites compared to TAS3, a single copy of ta-siARF, and a sequence conservation pattern pointing to the possibility that processing of novel TAS-like locus is initiated by cleavage of the 5'-terminal miR390 target site.lld:pubmed
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pubmed-article:19859540pubmed:authorpubmed-author:OzerovaL VLVlld:pubmed
pubmed-article:19859540pubmed:authorpubmed-author:MorozovS YSYlld:pubmed
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pubmed-article:19859540pubmed:articleTitleNovel miR390-dependent transacting siRNA precursors in plants revealed by a PCR-based experimental approach and database analysis.lld:pubmed
pubmed-article:19859540pubmed:affiliationInstitute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences, Timiryazevskaya 42, 127550 Moscow, Russia.lld:pubmed
pubmed-article:19859540pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19859540pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed