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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2009-10-27
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pubmed:abstractText |
TAS loci in plant genomes encode transacting small interfering RNAs (ta-siRNAs) that regulate expression of a number of genes. The function of TAS3 precursor in Arabidopsis thaliana is controlled by two miR390 target sites flanking two ta-siARF sequences targeting mRNAs of ARF transcription factors. Cleavage of the 3'-miR390-site initiates ta-siRNAs biogenesis. Here we describe the new method for identification of plant ta-siRNA precursors based on PCR with oligodeoxyribonucleotide primers mimicking miR390. The method was found to be efficient for dicotiledonous plants, cycads, and mosses. Based on sequences of amplified loci and a database analysis, a novel type of miR390-dependent TAS sequences was identified in dicots. These TAS loci are characterized by a smaller distance between miR390 sites compared to TAS3, a single copy of ta-siARF, and a sequence conservation pattern pointing to the possibility that processing of novel TAS-like locus is initiated by cleavage of the 5'-terminal miR390 target site.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19859540-15372043,
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1110-7251
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
2009
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
952304
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pubmed:dateRevised |
2010-9-28
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pubmed:meshHeading |
pubmed-meshheading:19859540-Arabidopsis,
pubmed-meshheading:19859540-Base Sequence,
pubmed-meshheading:19859540-DNA, Plant,
pubmed-meshheading:19859540-DNA Primers,
pubmed-meshheading:19859540-Databases, Nucleic Acid,
pubmed-meshheading:19859540-MicroRNAs,
pubmed-meshheading:19859540-Molecular Sequence Data,
pubmed-meshheading:19859540-Plants,
pubmed-meshheading:19859540-Polymerase Chain Reaction,
pubmed-meshheading:19859540-RNA, Plant,
pubmed-meshheading:19859540-RNA, Small Interfering,
pubmed-meshheading:19859540-RNA Precursors,
pubmed-meshheading:19859540-Sequence Homology, Nucleic Acid,
pubmed-meshheading:19859540-Species Specificity,
pubmed-meshheading:19859540-Tobacco,
pubmed-meshheading:19859540-Transcriptional Activation
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pubmed:year |
2009
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pubmed:articleTitle |
Novel miR390-dependent transacting siRNA precursors in plants revealed by a PCR-based experimental approach and database analysis.
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pubmed:affiliation |
Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences, Timiryazevskaya 42, 127550 Moscow, Russia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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