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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2009-12-16
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pubmed:abstractText |
Synphilin-1 is a cytoplasmic protein with unclear function. Synphilin-1 has been identified as an interaction partner of alpha-synuclein. The interaction between synphilin-1 and alpha-synuclein has implications in Parkinson's disease. In this study, we stably overexpressed human synphilin-1 in mouse N1E-115 neuroblastoma cells. We found that overexpression of synphilin-1 shortened cell growth doubling time and increased neurite outgrowth. Knockdown of endogenous synphilin-1 caused neuronal toxicity and shortened neurite outgrowth. We further found that synphilin-1 increased activation of the extracellular signal-regulated kinases (ERK1/2) and mediated neurite outgrowth. Rotenone, mitochondrial complex I inhibitor, has been shown previously to induce dopaminergic neurodegeneration and Parkinsonism in rats and Drosophila. We found that Rotenone induced apoptotic cell death in N1E-115 cells via caspase-3 activation and poly (ADP-ribose) polymerase (PARP) cleavage. Overexpression of synphilin-1 significantly reduced Rotenone-induced cell death, caspase-3 activation and PARP cleavage. The results indicate that synphilin-1 displays trophic and protective effects in vitro, suggesting that synphilin-1 may play a protective role in Parkinson's disease (PD) pathogenesis and may lead to a potential therapeutic target for PD intervention.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Casp3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Electron Transport Complex I,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Parp1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Poly(ADP-ribose) Polymerases,
http://linkedlifedata.com/resource/pubmed/chemical/Rotenone,
http://linkedlifedata.com/resource/pubmed/chemical/SNCAIP protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Sncaip protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1873-7544
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
20
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pubmed:volume |
165
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
455-62
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pubmed:meshHeading |
pubmed-meshheading:19857556-Animals,
pubmed-meshheading:19857556-Apoptosis,
pubmed-meshheading:19857556-Carrier Proteins,
pubmed-meshheading:19857556-Caspase 3,
pubmed-meshheading:19857556-Cell Enlargement,
pubmed-meshheading:19857556-Cell Line, Tumor,
pubmed-meshheading:19857556-Cell Proliferation,
pubmed-meshheading:19857556-Electron Transport Complex I,
pubmed-meshheading:19857556-Gene Knockdown Techniques,
pubmed-meshheading:19857556-Humans,
pubmed-meshheading:19857556-Mice,
pubmed-meshheading:19857556-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:19857556-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:19857556-Nerve Tissue Proteins,
pubmed-meshheading:19857556-Neurites,
pubmed-meshheading:19857556-Neurons,
pubmed-meshheading:19857556-Poly(ADP-ribose) Polymerases,
pubmed-meshheading:19857556-Rotenone,
pubmed-meshheading:19857556-Time Factors,
pubmed-meshheading:19857556-Transfection
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pubmed:year |
2010
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pubmed:articleTitle |
Synphilin-1 exhibits trophic and protective effects against Rotenone toxicity.
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pubmed:affiliation |
Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi, PR China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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