Linked Life Data

19857554

Source:http://linkedlifedata.com/resource/pubmed/id/19857554

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-2-1
pubmed:abstractText
We have studied brain stem cells in the ewe brain that project to the bed nucleus of the stria terminalis (BNST) and determined if these cells are activated by estradiol-17beta. This would predicate an indirect role in the estradiol-17beta regulation of gonadotropin releasing hormone (GnRH) cells, since these receive input from the BNST. Ovariectomized ewes received 50 mug estradiol-17beta benzoate (i.m.) 1 h prior to brain collection, so that activated cells could be identified by Fos immunohistochemistry. Retrograde tracer (FluoroGold; FG), was injected into the three divisions of the BNST and labeled cells were mapped to the A1 and A2 regions and the parabrachial nucleus (PBN) of the brain stem. With FG injection into the dorsal and lateral BNST, all FG-containing cells in the caudal A1 and 45% of those in A2 stained for dopamine-beta-hydroxylase (DBH), indicating noradrenergic type. No FG-labelled cells in the PBN were DBH-positive. In A1 and A2 respectively, 42% and 46% of FG-labelled cells were Fos-positive, with no double-labeling in cells of the PBN. In ewes receiving FG injections into the ventral BNST, estrogen receptor (ER)alpha-immunoreactive nuclei were found in 82% of A1-FG labeled and 38% of A2-FG labeled cells. No FG-labelled cells of the PBN were ERalpha-positive. Anterograde tracing from A1 with microruby injection identified projections to the PBN, BNST and preoptic area (POA). Thus, A1 and A2 noradrenergic neurons project to the BNST in the ewe brain, express ERalpha and are activated by estradiol-17beta. These noradrenergic, estrogen-responsive cells may provide indirect input to GnRH cells, via the BNST.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1873-7544
pubmed:author
pubmed:copyrightInfo
Copyright 2010 IBRO. Published by Elsevier Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
3
pubmed:volume
165
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
758-73
pubmed:meshHeading
pubmed-meshheading:19857554-Animals, pubmed-meshheading:19857554-Brain Stem, pubmed-meshheading:19857554-Cell Nucleus, pubmed-meshheading:19857554-Dopamine beta-Hydroxylase, pubmed-meshheading:19857554-Estradiol, pubmed-meshheading:19857554-Estrogen Receptor alpha, pubmed-meshheading:19857554-Estrogens, pubmed-meshheading:19857554-Female, pubmed-meshheading:19857554-Gonadotropin-Releasing Hormone, pubmed-meshheading:19857554-Immunohistochemistry, pubmed-meshheading:19857554-Neural Pathways, pubmed-meshheading:19857554-Neuronal Tract-Tracers, pubmed-meshheading:19857554-Ovariectomy, pubmed-meshheading:19857554-Preoptic Area, pubmed-meshheading:19857554-Proto-Oncogene Proteins c-fos, pubmed-meshheading:19857554-Receptors, Adrenergic, pubmed-meshheading:19857554-Septal Nuclei, pubmed-meshheading:19857554-Sheep
pubmed:year
2010
pubmed:articleTitle
Estradiol-17beta-responsive A1 and A2 noradrenergic cells of the brain stem project to the bed nucleus of the stria terminalis in the ewe brain: a possible route for regulation of gonadotropin releasing hormone cells.
pubmed:affiliation
Department of Physiology, Building 13F, Monash University, Clayton, VIC 3800, Australia.
pubmed:publicationType
Journal Article