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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2010-1-20
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pubmed:abstractText |
G2A is a G protein-coupled receptor that can be induced by various stressors. G2A is reported to have proton-sensing activity that mediates intracellular inositol phosphate (IP) accumulation with decreasing pH. We previously showed that G2A is also activated by some oxidized free fatty acids such as 9-hydroxyoctadecadienoic acid (9-HODE). In this study, we identified a novel alternative splice variant of G2A (G2A-b) that has a partially different N terminus compared with the G2A originally reported (G2A-a). The two splice variants of G2A show similar tissue distributions, but G2A-b is expressed more abundantly. There was no difference between the two variants in 9-HODE-induced cellular responses, such as intracellular calcium mobilization and GDP/GTP exchange of Galpha protein, and in proton-sensitive IP accumulation. However, G2A-b showed a higher basal activity in terms of IP accumulation. Mutagenesis study revealed that the difference in the basal activity is attributable to the K7 residue that exists only in G2A-a. We further demonstrated that an R42A mutation largely impaired both the basal and proton-sensing activities, but did not affect the 9-HODE-induced intracellular calcium increase. Taken together, we found an additional novel G2A variant (G2A-b) that is the major transcript with functional response to ligand stimulation as well as G2A-a, and succeeded in discriminating proton-sensing and oxidized fatty acid-sensing activities of G2A.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/9-hydroxy-10,12-octadecadienoic acid,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/G2A receptor,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Linoleic Acids, Conjugated,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1521-0103
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
332
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
469-78
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pubmed:meshHeading |
pubmed-meshheading:19855098-Alternative Splicing,
pubmed-meshheading:19855098-Amino Acid Sequence,
pubmed-meshheading:19855098-Animals,
pubmed-meshheading:19855098-CHO Cells,
pubmed-meshheading:19855098-COS Cells,
pubmed-meshheading:19855098-Calcium,
pubmed-meshheading:19855098-Cell Cycle Proteins,
pubmed-meshheading:19855098-Cercopithecus aethiops,
pubmed-meshheading:19855098-Cricetinae,
pubmed-meshheading:19855098-Cricetulus,
pubmed-meshheading:19855098-Cyclic AMP Response Element-Binding Protein A,
pubmed-meshheading:19855098-Guanosine Triphosphate,
pubmed-meshheading:19855098-HL-60 Cells,
pubmed-meshheading:19855098-Humans,
pubmed-meshheading:19855098-Inositol Phosphates,
pubmed-meshheading:19855098-Leukocytes,
pubmed-meshheading:19855098-Linoleic Acids, Conjugated,
pubmed-meshheading:19855098-Molecular Sequence Data,
pubmed-meshheading:19855098-Protein Isoforms,
pubmed-meshheading:19855098-RNA, Messenger,
pubmed-meshheading:19855098-Receptors, G-Protein-Coupled,
pubmed-meshheading:19855098-Serum Response Element,
pubmed-meshheading:19855098-Transfection
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pubmed:year |
2010
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pubmed:articleTitle |
Identification and analysis of two splice variants of human G2A generated by alternative splicing.
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pubmed:affiliation |
Department of Biochemistry, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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