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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2009-10-26
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pubmed:abstractText |
Orthologs of the yeast telomere protein Stn1 are present in plants, but other components of the Cdc13/Stn1/Ten1 (CST) complex have only been found in fungi. Here we report the identification of conserved telomere maintenance component 1 (CTC1) in plants and vertebrates. CTC1 encodes an approximately 140 kDa telomere-associated protein predicted to contain multiple OB-fold domains. Arabidopsis mutants null for CTC1 display a severe telomere deprotection phenotype accompanied by a rapid onset of developmental defects and sterility. Telomeric and subtelomeric tracts are dramatically eroded, and chromosome ends exhibit increased G overhangs, recombination, and end-to-end fusions. AtCTC1 both physically and genetically interacts with AtSTN1. Depletion of human CTC1 by RNAi triggers a DNA damage response, chromatin bridges, increased G overhangs, and sporadic telomere loss. These data indicate that CTC1 participates in telomere maintenance in diverse species and that a CST-like complex is required for telomere integrity in multicellular organisms.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1097-4164
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
23
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pubmed:volume |
36
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
207-18
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pubmed:dateRevised |
2011-6-15
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pubmed:meshHeading |
pubmed-meshheading:19854131-Anaphase,
pubmed-meshheading:19854131-Arabidopsis,
pubmed-meshheading:19854131-Arabidopsis Proteins,
pubmed-meshheading:19854131-Cell Line, Tumor,
pubmed-meshheading:19854131-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:19854131-Chromosomes, Plant,
pubmed-meshheading:19854131-Conserved Sequence,
pubmed-meshheading:19854131-Eukaryotic Cells,
pubmed-meshheading:19854131-Genomic Instability,
pubmed-meshheading:19854131-Humans,
pubmed-meshheading:19854131-In Situ Hybridization, Fluorescence,
pubmed-meshheading:19854131-Mutation,
pubmed-meshheading:19854131-Nucleic Acid Conformation,
pubmed-meshheading:19854131-Protein Binding,
pubmed-meshheading:19854131-Recombination, Genetic,
pubmed-meshheading:19854131-Telomere,
pubmed-meshheading:19854131-Telomere-Binding Proteins
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pubmed:year |
2009
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pubmed:articleTitle |
Conserved telomere maintenance component 1 interacts with STN1 and maintains chromosome ends in higher eukaryotes.
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pubmed:affiliation |
Department of Biochemistry and Biophysics, Texas A&M University, 2128 TAMU, College Station, TX 77843-2128, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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