19852763

Source:http://linkedlifedata.com/resource/pubmed/id/19852763

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0012854, umls-concept:C0034760, umls-concept:C0747055, umls-concept:C1521871, umls-concept:C2349975
pubmed:issue	3
pubmed:dateCreated	2009-10-26
pubmed:abstractText	Amplification of GC-rich DNA sequences is still a difficult task worldwide. Two frequently seen and inexpensive reagents-ethylene glycol and 1,2-propanediol-were found to be more effective than betaine in the amplification of 104 randomly selected GC-rich human DNA sequences with GC contents of 60-80% and lengths of 700-800 bp.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/8306785
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Ethylene Glycol, http://linkedlifedata.com/resource/pubmed/chemical/Propylene Glycol
pubmed:status	MEDLINE
pubmed:month	Sep
pubmed:issn	1940-9818
pubmed:author	pubmed-author:DadoDD, pubmed-author:MengLiangyuL, pubmed-author:ShenCenchaoC, pubmed-author:YangWenjuanW, pubmed-author:YangXiaX, pubmed-author:ZhangZhizhouZ
pubmed:issnType	Electronic
pubmed:volume	47
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	775-9
pubmed:meshHeading	pubmed-meshheading:19852763-Ethylene Glycol, pubmed-meshheading:19852763-GC Rich Sequence, pubmed-meshheading:19852763-Humans, pubmed-meshheading:19852763-Nucleic Acid Amplification Techniques, pubmed-meshheading:19852763-Propylene Glycol, pubmed-meshheading:19852763-Sequence Analysis, DNA
pubmed:year	2009
pubmed:articleTitle	Enhanced amplification of GC-rich DNA with two organic reagents.
pubmed:affiliation	HIT Bio-X Centre for Systems Biotechnology, Harbin Institute of Technology, Weihai, China. zhangzzbiox@gmail.com
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't