Linked Life Data

198491

Source:http://linkedlifedata.com/resource/pubmed/id/198491

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1977-11-30
pubmed:abstractText
An enzyme-linked immunosorbent assay (ELISA) was used for the detection in human sera of antibody to herpes simplex virus antigens. Development and standardization of the assay suggested that antigenic purity, temperature of reactions, concentration of enzyme-conjugated antiglobulin, and concentrations of test sera are all critical parameters of a successful ELISA procedure. When ELISA titers of 30 human sera were compared to micro-complement fixation titers of the same sera, a significant degree of correlation was observed, but quantitatively ELISA was found to be up to 200 times more sensitive. The sensitivity of the assay and its adaptability to automation should provide an additional method for study or diagnosis of infection with herpes simplex virus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-1899
pubmed:author
pubmed:issnType
Print
pubmed:volume
136 Suppl
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
S286-93
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Detection of antibodies specific for herpes simplex virus in human sera by the enzyme-linked immunosorbent assay.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.