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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0017262,
umls-concept:C0021585,
umls-concept:C0036536,
umls-concept:C0036537,
umls-concept:C0042711,
umls-concept:C0596260,
umls-concept:C0597879,
umls-concept:C0935640,
umls-concept:C1171362,
umls-concept:C1515670,
umls-concept:C1709694
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pubmed:issue |
1
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pubmed:dateCreated |
1991-1-29
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pubmed:abstractText |
Two recombinant baculoviruses were constructed by inserting via the transfer vector pAcYM1 the genes coding for the structural proteins of dengue (DEN)-2 virus downstream from the polyhedrin promoter of Autographa californica nuclear polyhedrosis virus. The two recombinants differed in truncation of 26 and 71 amino acids, respectively, in the carboxy-terminal sequence of DEN-specific envelope (E) glycoprotein. Recombinant DEN-2 E glycoproteins were processed and transported to the surface of Spodoptera frugiperda Sf9 cells infected with both viruses. We show that about one-third of the E glycoprotein minus its whole C-terminal hydrophobic anchor domain was secreted into an endoglycosidase H-resistant form. The type-specific neutralizing epitopes were conserved in the recombinant proteins as shown with a panel of monoclonal antibodies.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0042-6822
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
180
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
442-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1984665-Animals,
pubmed-meshheading:1984665-Antibodies, Monoclonal,
pubmed-meshheading:1984665-Antibodies, Viral,
pubmed-meshheading:1984665-Baculoviridae,
pubmed-meshheading:1984665-Base Sequence,
pubmed-meshheading:1984665-Biological Transport, Active,
pubmed-meshheading:1984665-Chromosome Deletion,
pubmed-meshheading:1984665-Cloning, Molecular,
pubmed-meshheading:1984665-DNA, Viral,
pubmed-meshheading:1984665-Dengue Virus,
pubmed-meshheading:1984665-Fluorescent Antibody Technique,
pubmed-meshheading:1984665-Gene Expression,
pubmed-meshheading:1984665-Humans,
pubmed-meshheading:1984665-Kinetics,
pubmed-meshheading:1984665-Molecular Sequence Data,
pubmed-meshheading:1984665-Moths,
pubmed-meshheading:1984665-Plasmids,
pubmed-meshheading:1984665-Protein Processing, Post-Translational,
pubmed-meshheading:1984665-Recombinant Fusion Proteins,
pubmed-meshheading:1984665-Viral Envelope Proteins
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pubmed:year |
1991
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pubmed:articleTitle |
Processing, secretion, and immunoreactivity of carboxy terminally truncated dengue-2 virus envelope proteins expressed in insect cells by recombinant baculoviruses.
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pubmed:affiliation |
Laboratoire des Arbovirus, Institut Pasteur, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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