Linked Life Data

19845106

Source:http://linkedlifedata.com/resource/pubmed/id/19845106

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-10-22
pubmed:abstractText
Standardized protocols and methods for virological monitoring are mandatory for the correct surveillance of human cytomegalovirus (HCMV) infection in transplanted patients receiving pre-emptive therapy. Fifteen Italian viral diagnostic laboratories belonging to different transplantation centers participated in the external Quality Control Programme for Molecular Diagnostics of HCMV-DNA by using two in-house and five commercial methods for HCMV-DNA quantification. The different methods shared 100% specificity, and sensitivity reached 100% when samples containing > 1,000 copies/ml were considered. The variability range was wide (about 2 log10) for samples containing a lower amount of HCMV-DNA (< 1,000 copies/ml), but it decreased with increasing concentrations of HCMV-DNA. For HCMV-DNA levels > or = 5,000 copies/ml, the different methods provided results within a +/- 0.5 log10 variability range, while the 80% range (range in which 80% of results obtained will fall) was within +/- 0.3 log10 or less. An acceptable level of variability was reached among different in-house and commercial methods for HCMV-DNA quantification in samples containing a clinically significant viral DNA amount. Based on these data, standardized cutoffs established for pre-emptive therapy in different transplantation centers should provide comparable clinical and virological results among centers.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1121-7138
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
245-53
pubmed:dateRevised
2011-9-13
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Multicenter quality control study for human cytomegalovirus DNAemia quantification.
pubmed:affiliation
Servizio di Virologia, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Multicenter Study