Source:http://linkedlifedata.com/resource/pubmed/id/19843882
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
25
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| pubmed:dateCreated |
2009-12-16
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| pubmed:abstractText |
The transcription factor Gata1 is required for the development of erythrocytes and megakaryocytes. Previous studies with a complementation rescue approach showed that the zinc finger domains are required for both primitive and definitive hematopoiesis. Here we report a novel zebrafish gata1 mutant with an N-ethyl-N-nitrosourea-induced point mutation in the C-finger (gata1(T301K)). The Gata1 protein with this mutation bound to its DNA target sequence with reduced affinity and transactivated inefficiently in a reporter assay. gata1(T301K/T301K) fish had a decreased number of erythrocytes during primitive hematopoiesis but normal adult hematopoiesis. We crossed the gata1(T301K/T301K) fish with those carrying the R339X mutation, also known as vlad tepes (vlt), which abolishes DNA binding and transactivation activities. As we reported previously, gata1(vlt/vlt) embryos were "bloodless" and died approximately 11 to 15 days after fertilization. Interestingly, the gata1(T301K/vlt) fish had nearly a complete block of primitive hematopoiesis, but they resumed hematopoiesis between 7 and 14 days after fertilization and grew to phenotypically normal fish with normal adult hematopoiesis. Our findings suggest that the impact of Gata1 on hematopoiesis correlates with its DNA-binding ability and that primitive hematopoiesis is more sensitive to reduction in Gata1 function than definitive hematopoiesis.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
1528-0020
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:day |
10
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| pubmed:volume |
114
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
5162-72
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| pubmed:dateRevised |
2011-10-7
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| pubmed:meshHeading |
pubmed-meshheading:19843882-Amino Acid Sequence,
pubmed-meshheading:19843882-Animals,
pubmed-meshheading:19843882-Binding Sites,
pubmed-meshheading:19843882-DNA,
pubmed-meshheading:19843882-Embryo, Nonmammalian,
pubmed-meshheading:19843882-Female,
pubmed-meshheading:19843882-Flow Cytometry,
pubmed-meshheading:19843882-GATA1 Transcription Factor,
pubmed-meshheading:19843882-Gene Expression Regulation, Developmental,
pubmed-meshheading:19843882-Hematopoiesis,
pubmed-meshheading:19843882-In Situ Hybridization,
pubmed-meshheading:19843882-Male,
pubmed-meshheading:19843882-Models, Molecular,
pubmed-meshheading:19843882-Molecular Sequence Data,
pubmed-meshheading:19843882-Mutation,
pubmed-meshheading:19843882-Nucleic Acid Conformation,
pubmed-meshheading:19843882-Protein Binding,
pubmed-meshheading:19843882-Protein Structure, Tertiary,
pubmed-meshheading:19843882-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19843882-Sequence Homology, Amino Acid,
pubmed-meshheading:19843882-Transcriptional Activation,
pubmed-meshheading:19843882-Zebrafish,
pubmed-meshheading:19843882-Zebrafish Proteins
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| pubmed:year |
2009
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| pubmed:articleTitle |
Differential requirement for Gata1 DNA binding and transactivation between primitive and definitive stages of hematopoiesis in zebrafish.
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| pubmed:affiliation |
Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Intramural
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