Linked Life Data

19842833

Source:http://linkedlifedata.com/resource/pubmed/id/19842833

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2009-10-21
pubmed:abstractText
To identify a proliferation-inducing ligand (APRIL) expression profile in tumor tissue and sputum of lung cancer, and evaluate the possibility of an assistant diagnosis of lung cancer by real-time fluorescence quantitative polymerase chain reaction (RTQ-PCR) in sputum as well, the authors analyzed the expression of APRIL mRNA in 75 tissue samples and 71 corresponding sputum samples of lung cancer by RTQ-PCR and analyzed their correlation. APRIL protein expression was also observed in tumor tissues by Western blot and immunohistochemistry. The expression analysis revealed APRIL expression was elevated in non-small cell lung cancer (NSCLC) and the expression of APRIL protein was located in the membrane and cytoplasm of tumor cells by immunohistochemiscal staining. Compared to benign pulmonary disease and healthy volunteers, the expression of APRIL mRNA in sputum of lung cancer was elevated (both P <. 001). When cut-off values for positivity were set at the mean + 2SD of mRNA expression in healthy volunteers, the positive rate for APRIL mRNA expression was 81.7% (58/71) in sputum samples of lung cancer, 3.2% (2/62) in benign pulmonary disease, and 1.5% (1/65) in healthy volunteers. The correlation was evident between the expression level of APRIL mRNA of tissue samples and that of sputum samples (P <. 001, r =. 702). These results support the possibility that the APRIL gene may play a key role in lung cancer, especially in NSCLC. The elevated expression level of APRIL mRNA in sputum of NSCLC suggested that APRIL mRNA may serve as an effective and convenient diagnostic biomarker for NSCLC.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1521-0499
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
486-500
pubmed:meshHeading
pubmed-meshheading:19842833-Aged, pubmed-meshheading:19842833-Base Sequence, pubmed-meshheading:19842833-Carcinoma, Non-Small-Cell Lung, pubmed-meshheading:19842833-Case-Control Studies, pubmed-meshheading:19842833-DNA Primers, pubmed-meshheading:19842833-Female, pubmed-meshheading:19842833-Gene Expression Profiling, pubmed-meshheading:19842833-Genetic Markers, pubmed-meshheading:19842833-Humans, pubmed-meshheading:19842833-Immunohistochemistry, pubmed-meshheading:19842833-Lung Diseases, pubmed-meshheading:19842833-Lung Neoplasms, pubmed-meshheading:19842833-Male, pubmed-meshheading:19842833-Middle Aged, pubmed-meshheading:19842833-RNA, Messenger, pubmed-meshheading:19842833-RNA, Neoplasm, pubmed-meshheading:19842833-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:19842833-Sputum, pubmed-meshheading:19842833-Tumor Markers, Biological, pubmed-meshheading:19842833-Tumor Necrosis Factor Ligand Superfamily Member 13
pubmed:year
2009
pubmed:articleTitle
A proliferation-inducing ligand: a new biomarker for non-small cell lung cancer.
pubmed:affiliation
Laboratory Medicine Center, Affiliated Hospital of Nantong University, Jiangsu Province, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't