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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1977-11-30
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pubmed:abstractText |
Y-1 adrenal cells responded to serum step down by a several fold decrease in DNA synthesis. Serum starved cells resumed DNA synthesis upon serum step up. ACTH and cAMP inhibited DNA synthesis both at low and high serum concentrations, a fact previously known. Pituitary, brain and liver crude extracts stimulated DNA synthesis in serum starved cells. Purified pituitary factors preparations contained two activities: one specific for Y-1 cells and another active with both fibroblasts and Y-1 cells. The kinetics of restimulation of DNA synthesis by serum and pituitary factors was studied. DNA synthesis restimulation occurred after a lag of 11 hours. This lag did not vary irrespective of the type of stimulator or its concentration. Cells entered S phase continuously at a rate which increased with increasing concentrations of the stimulator. Cells became refractory to the inhibitory action of ACTH five hours before entering S phase. The implications of these data to the understanding of cell growth control are considered.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adrenocorticotropic Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrocortisone,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Liver Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/Tissue Extracts
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9541
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-9
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:198417-Adrenocorticotropic Hormone,
pubmed-meshheading:198417-Blood,
pubmed-meshheading:198417-Brain,
pubmed-meshheading:198417-Cell Cycle,
pubmed-meshheading:198417-Cell Line,
pubmed-meshheading:198417-Cyclic AMP,
pubmed-meshheading:198417-DNA,
pubmed-meshheading:198417-Hydrocortisone,
pubmed-meshheading:198417-Insulin,
pubmed-meshheading:198417-Liver Extracts,
pubmed-meshheading:198417-Pituitary Gland,
pubmed-meshheading:198417-Tissue Extracts
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pubmed:year |
1977
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pubmed:articleTitle |
On the regulation of DNA synthesis in a line of adrenocortical tumor cells: effect of serum, adrenocorticotropin and pituitary factors.
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pubmed:publicationType |
Journal Article
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